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E-GEOD-109644

ABSTRACT: Pathogen Trojan horse delivers bioactive host protein ZmMAC1 to alter maize (Zea mays) anther cell behaviour in the male sterile mutant Zmmac1-1.

PROVIDER: E-GEOD-109644 | ExpressionAtlas | 2020-04-06

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-109644-analysis-methods.tsv	Tabular
	E-GEOD-109644-analytics.tsv	Tabular
	E-GEOD-109644-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-109644-configuration.xml	Xml
	E-GEOD-109644-g1_g2-mvaPlot.png	Other

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Pathogen Trojan horse delivers bioactive host protein ZmMAC1 to alter maize (Zea mays) anther cell behavior in the male sterile mutant Zmmac1-1.

Project description:Pathogen Trojan horse delivers bioactive host protein ZmMAC1 to alter maize (Zea mays) anther cell behavior in the male sterile mutant Zmmac1-1.

| PRJNA431598 | ENA

Transcriptome profiling of maize anthers using genetic ablation to analyze pre-meiotic and tapetal cell types

Project description:Agilent oligonucleotide arrays were used to profile gene expression in dissected maize anthers of 3 types of male-sterile plants and their fertile siblings at four stages of development: after anther initiation, at the rapid mitotic proliferation stage, pre-meiosis, and meiotic prophase I. The male-sterile mutants (ms23, msca, and mac1) lack a range of normal cell types resulting from a temporal progression of anther failure. By combining the data sets from the comparisons between individual sterile and fertile anthers, candidate genes predicted to play important roles during maize anther development were assigned to stages and to likely cell types. Keywords: anther development, maize, male sterility

2008-09-16 | GSE12756 | GEO

Transcriptome profiling of maize anthers using genetic ablation to analyze pre-meiotic and tapetal cell types

2008-09-16 | E-GEOD-12756 | biostudies-arrayexpress

The male sterility 8 mutation of maize disrupts the temporal progression of the transcriptome and results in the mis-regulation of metabolic functions

Project description:Phenotypes of maize male sterile 8 plants are small anthers, meiotic failure, shorter epidermal cells, and non-secreting tapetal cells. Excess callose accumulates around meiotic cells, which later abort. Thousands of transcriptome changes occur including ectopic activation of genes not expressed in fertile siblings, failure to express genes, and differential expression of genes shared with fertile siblings. Sixty-three differentially expressed proteins were identified after 2-D DIGE followed by LC/MS/MS, including those involved in metabolism and cell division. The majority were not identified by differential RNA expression. Keywords: anther development, maize, male-sterile, ms8 4 replicates of ms8 mutant and 4 replicates of ms8 fertile siblings at 3 anther stages. Spike-in controls were included.

2010-08-20 | E-GEOD-23615 | biostudies-arrayexpress

Transcriptome analysis of male flowers corresponding to anther developmental stages in Cryptomeria japonica

Project description:Investigation of gene expression changes among anther development in wild type and male sterile mutant.

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Transcriptome analysis of multiple pre-meiotic anther stages and laser microdissected cell types in maize

Project description:Transcriptomes from multiple pre-meiotic stages of wild type, mac1, and msca1 maize anthers were characterized by microarray hybridization. The goal was to characterize the developmental progression as the anther specifies five cell types and grows rapidly precedeing meiotic entry. The stages characterized were immature anther primordia (0.15 mm long in maize) containing just stem cells, through somatic and germinal cell fate specification (0.20 and 0.25 mm), mitotic proliferation (0.4 mm), and finally the birth of the middle layer and tapetum (0.7 mm). To obtain cell-type specific markers, at 0.7 mm we also compared whole anthers to collections of laser-microdissected anther cell types including the archesporial cells (pre-meiotic germinal cells), nutritive layers (middle layer and tapetum) and structural layers (endothecium and epidemis) of the anther lobe. keyword: anther development, maize, male-sterile

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Transcriptome profiling of maize anthers infected by Ustilago maydis

Project description:Two-organism transcriptome profiling of infected maize anthers demonstrated that there are anther-specific gene expression programs for both the pathogen and the host. Two-dye, competitive microarray hybridizations were performed on Agilent oligo arrays. Confocal microscopy on other anthers demonstrated that U. maydis was in contact with all anther lobe cell types by 3 days post-infection and that the pathogen disrupted both host cell division and cell expansion patterns.

2013-03-21 | GSE43544 | GEO

Global transcriptome analysis of two ameiotic1 alleles in maize anthers: defining steps in meiotic entry and progression through prophase

Project description:This study compared the transcriptomes of maize anthers at both 1mm and 1.5mm lengths from male sterile mutants with either the am1-489 or am1-pra alleles as well as from fertile siblings of the mutant plants. Comparisons were done varying just the stage (1mm vs 1.5 mm), just the allele (am1-pra vs am1-489 at the same stage), and wild-type vs mutant (same stage and allele). Genes were categorized as On (expressed) or Off (not detected) and if both channels produced an intensity, the differential expression was determined. AM1 seems to be involved in establishing or stabilizing meiotic chromosomes and it had a profound impact on gene expression in the pollen mother cells during the maturation phase from 1.0 to 1.5 mm anther length. The two alleles shared some transcript changes but am1-489 showed a more dramatic loss (>3400) at 1.5mm than am1-pra. Most differentially expressed transcripts were allele-specific. KEYWORDS: male sterility, maize, microarray, meiosis Compared wild-type and male-sterile anthers from 2 ameiotic1 alleles at 2 stages of anther growth (1mm and 1.5mm)

2011-07-14 | E-GEOD-30149 | biostudies-arrayexpress

Transcriptome analysis of male sterile maize mutants defective in somatic cell layer identities (tcl1, mtm00-06 and ms*6015) compared with fertile siblings

Project description:In order to better understand defects at the molecular level in male sterile maize mutants, we characterized 3 different mutants by microarray hybridization. Anthers were dissected and staged to be 1000 Î¼m in length (+/- 100 Î¼m), allowing us to analyze changes in transcriptome between siblings relatively close to the initial observation of morphological defects. All three of the mutants had morphological defects in the somatic layers of the anther, but only tcl1 and mtm00-06 male steriles show serial extra periclinal divisions compared to relatively minor defects in ms*6015 male steriles. For each mutant four biological replicates were used for both male sterile samples and fertile siblings with direct comparisons made between fertile and sterile siblings of the same mutant background.

2016-07-01 | E-GEOD-78259 | biostudies-arrayexpress

Hypoxia triggers meiotic fate acquisition in maize

Project description:Evidence from confocal microscopic reconstruction of maize anther development in fertile, mac1 (excess germ cells) and msca1 (no germ cells) flowers indicates that the male germ line is multiclonal and uses the MAC1 protein to organize the somatic niche. Furthermore, we identified redox status as a determinant of germ cell fate, defining a mechanism distinct from the animal germ cell lineage. Decreasing oxygen or H2O2 increases germ cell numbers, stimulates superficial germ cell formation, and rescues germinal differentiation in msca1 flowers. Conversly, oxidizing environments inhibit germ cell specification and cause ectopic differentiation in deeper tissues. We propose that hypoxia, arising naturally within growing anther tissue, acts as a positional cue to set germ cell fate. key words: anther development, maize, male-sterile

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