Transcriptome analysis of male flowers corresponding to anther developmental stages in Cryptomeria japonica
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ABSTRACT:
ORGANISM(S): Cryptomeria japonica
PROVIDER: GSE64663 | GEO | 2016/03/01
SECONDARY ACCESSION(S): PRJNA271594
REPOSITORIES: GEO
Similar Datasets
Project description:Investigation of gene expression changes among anther development in wild type and male sterile mutant. Male strobili of wild-type were collected at 10 stages with three biological repeats (total 30 samples) and male strobili of mutant were collected at stages 3 to 6 with three biological repeats (total 12 samples).
2016-03-01 | E-GEOD-64663 | biostudies-arrayexpress
Project description:Wheat, an important human food crop, is very sensitive to temperature changes. Thermosensitive sterile lines (TMS) is a natural material for exploring the effects of anther development and temperature changes on male fertility. In order to study the possible molecular mechanism of regulating protein activity during anther fertility alteration, proteomics analysis based on high resolution mass spectrometry were used to study the binuclear anther of wheat YS3038.
2022-08-12 | PXD029487 | Pride
Project description:The molecular mechanisms that control male strobilus development in conifers are largely unknown because the developmental stages and related genes have not yet been characterized. The determination of male strobilus developmental stages will contribute to genetic research and reproductive biology in conifers. Our objectives in this study were to determine the developmental stages of male strobili by cytological and transcriptome analysis, and to determine the stages at which aberrant morphology is observed in a male-sterile mutant of Cryptomeria japonica D. Don to better understand the molecular mechanisms that control male strobilus and pollen development. Male strobilus development was observed for 8 months, from initiation to pollen dispersal. A set of 19,209 expressed sequence tags (ESTs) collected from a male reproductive library and a pollen library was used for microarray analysis. We divided male strobilus development into 10 stages by cytological and transcriptome analysis. Eight clusters (7324 ESTs) exhibited major changes in transcriptome profiles during male strobili and pollen development in C. japonica Two clusters showed a gradual increase and decline in transcript abundance, respectively, while the other six clusters exhibited stage-specific changes. The stages at which the male sterility trait of Sosyun was expressed were identified using information on male strobilus and pollen developmental stages and gene expression profiles. Aberrant morphology was observed cytologically at Stage 6 (microspore stage), and differences in expression patterns compared with wild type were observed at Stage 4 (tetrad stage).
| S-EPMC4886286 | biostudies-literature
Project description:Dominant genic male sterility (DGMS) is a valuable system for hybrid seed production and improvement of plant populations, but the molecular mechanism of DGMS is not well understood. To facilitate the analysis of mechanism underlying DGMS, we compared gene expression profiles between fertile and sterile flowers of two pair of Brassica napus isogenic lines: J10AB and J03AB which only differ in sterility-related locus and fertility restore-related locus respectively by using Arabidopsis thaliana ATH-1 microarray. Gene expression profiling revealed that 360 genes differed in expression between the two lines and the number of differently expressed genes increased with progressing developmental stages. Cluster analysis result indicates that most important gene expression changes occur from PMC (anther less than 1mm) to uninucleate stage (anther in 3mm). There are 182 differential expressed genes detected between fertile plants and sterile plants at same stage. Some important pathways related to fertility control by KEGG analysis, such as glycolysis/gluconeogenesis pathway, flavonoid biosynthesis pathway and fatty acid metabolism pathway. Another abortion usually associated with suppression of gene expression of these pathways. Keywords: Time course analysis
2007-12-07 | GSE9721 | GEO
Project description:Investigation of comprehensive gene expression level changes in male strobili of sterile mutant, compared to the fertile plant. We designed a new custum array derived from ESTs of Cryptomeria japonica for this study. Male strobili of C. japonica were collected from Toyama 1 half-sib F2 pedigree. Toyama 1 male-sterile mutant was first reported in Saito, M. , H. Taira, and Y. Furuta (Journal of Forest Research 3(3):167-173).
2013-07-30 | GSE33499 | GEO
Project description:Investigation of comprehensive gene expression level changes in male strobili of sterile mutant, compared to the fertile plant. We designed a new custum array derived from ESTs of Cryptomeria japonica for this study. Male strobili of C. japonica were collected from Toyama 1 half-sib F2 pedigree. Toyama 1 male-sterile mutant was first reported in Saito, M. , H. Taira, and Y. Furuta (Journal of Forest Research 3(3):167-173). Microarray studies using both individual samples and pooled samples were performed. Total RNA was extracted from male strobili at four developmental stages derived from each seven fertile and male-sterile trees as individual samples. Pooled RNA samples were prepared from four to eight different fertile and sterile plants at three developmental stages.
2013-07-30 | E-GEOD-33499 | biostudies-arrayexpress
Project description:The RNA-seq was used to identify differentially regulated miRNAs between a male sterile and wild type tomato during anther development.
2015-10-01 | GSE65788 | GEO
Project description:Transcriptomes from multiple pre-meiotic stages of wild type, mac1, and msca1 maize anthers were characterized by microarray hybridization. The goal was to characterize the developmental progression as the anther specifies five cell types and grows rapidly precedeing meiotic entry. The stages characterized were immature anther primordia (0.15 mm long in maize) containing just stem cells, through somatic and germinal cell fate specification (0.20 and 0.25 mm), mitotic proliferation (0.4 mm), and finally the birth of the middle layer and tapetum (0.7 mm). To obtain cell-type specific markers, at 0.7 mm we also compared whole anthers to collections of laser-microdissected anther cell types including the archesporial cells (pre-meiotic germinal cells), nutritive layers (middle layer and tapetum) and structural layers (endothecium and epidemis) of the anther lobe. keyword: anther development, maize, male-sterile
2013-05-01 | GSE43982 | GEO
Project description:Dominant genic male sterility (DGMS) is a valuable system for hybrid seed production and improvement of plant populations, but the molecular mechanism of DGMS is not well understood. To facilitate the analysis of mechanism underlying DGMS, we compared gene expression profiles between fertile and sterile flowers of two pair of Brassica napus isogenic lines: J10AB and J03AB which only differ in sterility-related locus and fertility restore-related locus respectively by using Arabidopsis thaliana ATH-1 microarray. Gene expression profiling revealed that 360 genes differed in expression between the two lines and the number of differently expressed genes increased with progressing developmental stages. Cluster analysis result indicates that most important gene expression changes occur from PMC (anther less than 1mm) to uninucleate stage (anther in 3mm). There are 182 differential expressed genes detected between fertile plants and sterile plants at same stage. Some important pathways related to fertility control by KEGG analysis, such as glycolysis/gluconeogenesis pathway, flavonoid biosynthesis pathway and fatty acid metabolism pathway. Another abortion usually associated with suppression of gene expression of these pathways. Experiment Overall Design: The Homozygous DGMS two-type line J10AB was compared with heterozygous two-type line J03AB at three different stages of development: tetrad stage, uninucleate stage and trinucleate stage (Flower buds <1, 1-3 and >3mm, respectively) which were confirmed by cytology observation. All these flower buds were collected by hand. For each stage, two biological repetitions and two experimental repetitions were used, resulting in four hybridizations per developmental stage. Gene expression was compared between lines at different stages of development, as well as between developmental stages of fertile plants and sterile plants of each line.
2008-06-16 | E-GEOD-9721 | biostudies-arrayexpress
Project description:Transcriptome analysis of male flowers corresponding to anther developmental stages in Cryptomeria japonica
| PRJNA271594 | ENA