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E-GEOD-1934

ABSTRACT: Transcription profiling time series IFH1 overexpression by galactose induction in S. cerevisiae

PROVIDER: E-GEOD-1934 | ExpressionAtlas | 2015-09-08

REPOSITORIES: ExpressionAtlas

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Dataset's files

Source:

			Action	DRS
	E-GEOD-1934-analysis-methods.tsv	Tabular
	E-GEOD-1934-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-1934-configuration.xml	Xml
	E-GEOD-1934-percentile-ranks.tsv	Tabular
	E-GEOD-1934.condensed-sdrf.tsv	Tabular

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Project description:Experiment Design: •Type of experiment: time course after GAL (galactose) induction •Experimental factor: time, genotype •Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: •The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) •Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2% •Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen. Keywords = IFH1 Keywords = Transcriptional control of RP genes Keywords: time-course

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