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E-GEOD-19603

ABSTRACT: Transcription profiling by array of Arabidopsis msh1 recA3 double mutants under heat stress

OTHER RELATED OMICS DATASETS IN: PRJNA122437

PROVIDER: E-GEOD-19603 | ExpressionAtlas | 2015-11-19

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-19603-analysis-methods.tsv	Tabular
	E-GEOD-19603-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-19603-configuration.xml	Xml
	E-GEOD-19603-percentile-ranks.tsv	Tabular
	E-GEOD-19603.condensed-sdrf.tsv	Tabular

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Nontargeted and targeted metabolomics measurements of abiotic stress responses in three-week-old Arabidopsis thaliana plants' rosette leaf tissue

Project description:Nontargeted and targeted metabolomics measurements of abiotic stress responses in three-week-old Arabidopsis thaliana plants' rosette leaf tissue for Col-0 wild type plants and double/triple knockout mutants of aquaporins (pip2;1 pip2;2 and pip2;1 pip2;2 pip2;4) treated with drought, heat at different air humidities, or combined drought-heat stress at different air humidities. This experiment contains FT-ICR-MS measurements for 103 Arabidopsis thaliana rosette leaf samples covering three genotypes under six different environmental conditions. The three genotypes comprise the Col-0 wildtype and two loss-of-function mutants of aquaporins, a pip2;1 pip2;2 double mutant and a pip2;1 pip2;2 pip2;4 triple mutant (respective AGI locus identifiers: AT3G53420, AT2G37170, AT5G60660). The six conditions include control condition (well-watered, 22 °C, 70% relative air humidity), drought stress (one week without watering), heat stress without changing the absolute humidity of the ambient air (6 hours at 33 °C, 37% relative air humidity), heat stress with supplemented air humidity to maintain a constant vapor pressure deficit before and during the heat episode (6 hours at 33 °C, 84% relative air humidity), and the combinations of drought pretreatment with each of the two heat stress variants (one week of drought followed by 6 hours of heat stress). Samples from all conditions were harvested at the same time (within 15 min starting at 5 pm). For validation, GC-TOF-MS measurements were done for two genotypes (wildtype, double mutant) and two conditions (drought, control) on partially overlapping samples.

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'Microarray analysis of abiotic stress responses in three-week-old Arabidopsis thaliana plants'' rosette leaf tissue for Col-0 wild type plants and double/triple knockout mutants of aquaporins (pip2;1 pip2;2 and pip2;1 pip2;2 pip2;4) treated with drought, heat at different air humidities, or combined drought-heat stress at different air humidities'

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