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E-GEOD-38409

ABSTRACT: Expression data from mouse lungs, exposed in-utero to second-hand smoke (SHS) and challenged with ovalbumin (OVA) as adults.

OTHER RELATED OMICS DATASETS IN: PRJNA167827

PROVIDER: E-GEOD-38409 | ExpressionAtlas | 2015-09-28

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-38409-analysis-methods.tsv	Tabular
	E-GEOD-38409-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-38409-configuration.xml	Xml
	E-GEOD-38409-percentile-ranks.tsv	Tabular
	E-GEOD-38409.condensed-sdrf.tsv	Tabular

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Project description:Expression data from mouse lungs, exposed in-utero to second-hand smoke (SHS) and challenged with ovalbumin (OVA) as adults.

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Dissecting the molecular effects of cigarette smoke on proteasome function

Project description:Proteasome dysfunction is emerging as a novel pathomechanism for the development of chronic obstructive pulmonary disease (COPD), a major leading cause of death in the world. Cigarette smoke is one of the main risk factors for COPD and has been shown to impair proteasome function in vitro and in vivo. Importantly, proteasome activity is inhibited in COPD lungs while expression levels of proteasome subunits are not altered. In the present study, we dissected the molecular changes induced by cigarette smoke on proteasome function in lung epithelial cells and mouse lungs. We analyzed the integrity, composition, and the interactome of isolated 26S proteasome complexes from smoke-exposed cells and mouse lungs. Moreover, we applied native MS analysis to investigate whether reactive compounds of cigarette smoke directly modify and inhibit the 20S proteasome complex. Our data reveal that the 20S proteasome is slightly destabilized in the absence of any dominant modification of proteasomal proteins. 26S pulldown and stoichiometry analysis indicated that 26S proteasome complexes become instable in response to cigarette smoke exposure. Of note, the interactome of the 26S was clearly altered in smoke-exposed mouse lungs possibly reflecting an altered cellular composition in the lungs of the smoke-exposed mice. Taken together, our results suggest that cigarette smoke induces minor but detectable changes in the stability and interactome of 20S and 26S proteasome complexes which might contribute in a chronic setting to imbalanced proteostasis as observed in chronic lung diseases associated with cigarette smoking.

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Project description:Expression data from mouse lungs exposed in-utero and/or as an adult to second-hand smoke (SHS)

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E-GEOD-36810

Project description:Expression data from mouse lungs exposed in-utero and/or as an adult to second-hand smoke (SHS)

2015-09-28 | E-GEOD-36810 | ExpressionAtlas

Expression data from mouse lungs exposed in-utero and/or as an adult to second-hand smoke (SHS)

Project description:Second-hand smoke (SHS) exposure during pregnancy has adverse effects on offspring. We used microarrays to characterize the gene expression changes caused by in-utero exposure and adult exposure to SHS in adult mouse lungs. Left lungs from Balb/c male mice were collected at 15 weeks of age for RNA extraction and hybridization on Affymetrix mouse 430 2.0 microarrays. Based on their smoke exposure status, there are 4 groups of mice, each exposed in-utero to filtered-air or SHS and as an adult to filtered-air or SHS. We extracted RNA from 4 animals from each group for microarray analysis (N = 16 samples).

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Expression data from mouse lungs exposed in-utero and/or as an adult to second-hand smoke (SHS)

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Comparison analysis of 2 week old C57BL6J lung exposed to 2 weeks of cigarette smoke compared to age-matched controls

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