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E-GEOD-40824

ABSTRACT: SM019 - Infection with SARS MA15 of C57BL/6J mice and Tnfrsf1b knockout mice

PROVIDER: E-GEOD-40824 | ExpressionAtlas | 2020-03-31

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-40824-analysis-methods.tsv	Tabular
	E-GEOD-40824-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-40824-configuration.xml	Xml
	E-GEOD-40824-percentile-ranks.tsv	Tabular
	E-GEOD-40824.condensed-sdrf.tsv	Tabular

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SM019 - Infection with SARS MA15 of C57BL/6J mice and Tnfrsf1b knockout mice

Project description:Comparing the host response of C57BL/6J mice to Tnfrsf1b knockouts in the same strain of mice. Groups of 10-week-old C57BL/6 mice or Tnfrsf1a/1b knockout mice were infected with SARS MA15 at a dose of 10^5 PFU or mock infected. Mice were euthanized on days 4 and 7 post-infection to measure virus load and isolate samples for measurement of virus load, lung pathology, transcriptional analysis and proteomics analysis. Mock-infected animals were also harvested at each time point. Mice were weighed every 24 hours to measure general disease progression and any mouse approaching 30% weight loss was euthanized.

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