Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-GEOD-49016

ABSTRACT: Transcription profiling by array of monocyte-derived dendritic cells stimulated by intracellular bacteria (T. whipplei, B. abortus, C. burnetii, O. tsutsugamushi), lipopolysaccharide against unstimulated controls

OTHER RELATED OMICS DATASETS IN: PRJNA212694

PROVIDER: E-GEOD-49016 | ExpressionAtlas | 2015-03-16

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-GEOD-49016-analysis-methods.tsv	Tabular
	E-GEOD-49016-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-49016-configuration.xml	Xml
	E-GEOD-49016-percentile-ranks.tsv	Tabular
	E-GEOD-49016.condensed-sdrf.tsv	Tabular

Items per page:

1 - 5 of 116

Similar Datasets

Quantitative analysis of large-scale proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study

Project description:Determination of global alterations in monocyte-derived dendritic cells in response to stimulation with lipopolysaccharide using SWATH-MS to provide insight into cellular mechanism driving dendritic cell maturation

2019-03-18 | PXD008898 | Pride

moDCs HPLC-MS/MS - Unbiased Quantitative Proteomics Reveals a Crucial Role of the Allergen Context for the Activation of Human Dendritic Cells

Project description:To investigate the allergenicity of the major birch pollen allergen Bet v 1 and the impact of known adjuvants coming from pollen, such as lipopolysaccharide (LPS), we performed quantitative proteome analysis of stimulated monocyte-derived dendritic cells (moDCs). Thus, we treated cells with birch pollen extract (BPE), a recombinant variant of Bet v 1 or LPS followed by proteomic profiling by means of high performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS) using isobaric labelling.

2017-12-01 | PXD006196 | Pride

Expression data measured by Nanostring of monocyte-derived dendritic cells from healthy individuals stimulated with LPS, influenza, or IFN-beta, or left unstimulated

Project description:Variation in individuals' responses to environmental factors is believed to influence susceptibility to complex diseases in humans. The genetic basis of such variation is poorly understood. We measured gene expression from resting and stimulated dendritic cells (DCs) derived from the peripheral blood of healthy individuals. We stimulated the primary DCs with E. coli lipopolysaccharide (LPS), influenza virus or the cytokine IFNβ, and associated genetic variation between individuals with the observed variation in gene expression and gene induction. We collected peripheral blood from each human donor. We isolated peripheral blood mononuclear cells by Ficoll, and magnetically sorted them for CD14+CD16- monocytes. We then differentiated the monocytes into monocyte-derived dendritic cells (MoDCs) by culturing the cells for 7 days with GM-CSF and IL-4. We stimulated the cells with E. coli lipopolysaccharide (LPS) for 2.5 hr or 5 hr, influenza (PR8 dNS1) for 10 hr, or recombinant IFN-beta for 6.5 hr. Finally, we lysed the cells and ran Nanostring on the lysates.

2014-03-01 | E-GEOD-53165 | biostudies-arrayexpress

Expression data measured by microarray of monocyte-derived dendritic cells from healthy individuals stimulated with LPS, influenza, or left unstimulated

Project description:Variation in individuals' responses to environmental factors is believed to influence susceptibility to complex diseases in humans. The genetic basis of such variation is poorly understood. We measured gene expression from resting and stimulated dendritic cells (DCs) derived from the peripheral blood of healthy individuals. We stimulated the primary DCs with E. coli lipopolysaccharide (LPS) or influenza virus. Using serial replicate samples, we selected genes that showed evidence of reproducibility within the serial replicates. We collected peripheral blood from each human donor. We isolated peripheral blood mononuclear cells by Ficoll, and magnetically sorted them for CD14+CD16- monocytes. We then differentiated the monocytes into monocyte-derived dendritic cells (MoDCs) by culturing the cells for 7 days with GM-CSF and IL-4. We stimulated the cells with E. coli lipopolysaccharide (LPS) for 5 hr or influenza (PR8 dNS1) for 10 hr. Finally, we lysed the cells and isolated total RNA for microarray.

2014-03-01 | E-GEOD-53166 | biostudies-arrayexpress

Vitamin D modulation of innate immune signalling in Dendritic Cells

Project description:Vitamin D is widely reported to inhibit innate immune signalling and dendritic cell (DC) maturation, leading to attenuation of DC mediated T cell activation as a potential immunoregulatory mechanism to guard against autoimmunity and immmunopathology. It is not known whether vitamin D has global or gene specific effects on transcriptional responses downstream of innate immune stimulation. In order to address this question, we used genome-wide transcriptional profiling of monocyte derived DC differentiated in the presence and absence of vitamin D, and then stimulated with and without lipopolysaccharide for four hours.

2016-10-03 | E-MTAB-4883 | biostudies-arrayexpress

Genome-wide analysis of bone marrow-derived dendritic cells, unstimulated or stimulated with Bifidobacterium in vitro.

Project description:Comparison of gene expression in bone marrow-derived dendritic cells (DCs) unstimulated or stimulated with Bifidobacterium in vitro.

2015-11-12 | GSE73476 | GEO

Sumoylation-deficient bone marrow derived dendritic cells transcriptomic analysis after LPS stimulation

Project description:Bone marrow derived dendritic cells were generated from Ubc9[fl;-] and Ubc9[+/+] mice. After in vitro derivation in the presence of GM-CSF, dendritic cells were treated with tamoxifen for four days to cause CreERT2 activation, and induce Ubc9 floxed allele deletion. This allowed comparative transcriptomic analysis of Ubc9[+/+] and Ubc9[-/-] dendritic cells unstimulated or stimulated with 10ng/ml LPS for one hour and six hours. Three biological replicates of each conditions were used. Analyzed bone marrow derived dendritic cells were Ubc9 WT or Ubc9 KO. Cells were left unstimulated or stimulated with LPS 10 ng/ml for one hour and six hours.

2015-12-14 | E-GEOD-66178 | biostudies-arrayexpress

Transcription profiling by array of human monocyte derived dendritic cells stimulated by lipopolysaccharide (LPS), Mycobacterium vaccae (MV) and Pam3CSK4 (PCSK).

Project description:Comparison of human monocyte derived dendritic cell transcriptional responses to stimulation with lipopolysaccharide (LPS), Mycobacterium vaccae (MV) and Pam3CSK4 (PCSK)

2011-04-01 | E-TABM-998 | biostudies-arrayexpress

Paracrine activation of hCMEC/D3 cells by LPS-stimulated macrophages

Project description:Transcriptional profiling of hCMEC/D3 endothelial cells after six hour incubation with conditioned media from unstimulated or LPS-stimulated monocyte derived macrophages

2015-12-31 | E-MTAB-3692 | biostudies-arrayexpress

Genome-wide analysis of FcÎ³ receptor ligation on dendritic cells from wild-type, FcÎ³RIIb KO or FcR Î³ chain KO mice

Project description:Analysis of changes in gene expression after incubation of dendritic cells with immune complexes or medium. Since the dendritic cells are derived from three different mouse strains, either wild type, FcÎ³ receptor IIb KO (expresses only activating FcÎ³ receptors) or Fc receptor Î³ chain KO (expresses only inhibitory FcÎ³ receptor), the analysis gives important insight into the roles of the activating versus inhibiting FcÎ³ receptors on dendritic cells. Bone marrow-derived dendritic cells of the three mouse genotypes (see above) were incubated for 4 hours with medium (unstimulated) or OVA-anti OVA immune complexes (IC, stimulated) and changes in gene expression after stimulation with IC were compared between unstimulated vs stimulated with IC and across the three genotypes.

2012-05-02 | E-GEOD-33718 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data