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E-GEOD-6802

ABSTRACT: Transcription profiling by array of human bronchial epithelial cells infected with Pseudomonas aeruginosa, Staphylococcus aureus or respiratory syncytial virus

OTHER RELATED OMICS DATASETS IN: PRJNA99201

PROVIDER: E-GEOD-6802 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-6802-analysis-methods.tsv	Tabular
	E-GEOD-6802-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-6802-configuration.xml	Xml
	E-GEOD-6802-percentile-ranks.tsv	Tabular
	E-GEOD-6802-zoomifications-log.tsv	Tabular

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Transcription profiling of human bronchial airway epithelial cells infected with P. aeruginosa, S.aureus or respiratory syncytial virus vs uninfected controls to investigate the role of epithelial cells upon infection of airway pathogens

Project description:Bronchial epithelial cells represent the first line of defense against invading airborne pathogens. They are important contributors to innate mucosal immunity and provide a variety of anti-microbial effectors. To investigate the role of epithelial cells upon infection of airway pathogens, we stimulated BEAS-2B cells for 4 h with UV-inactivated bronchial pathogens including Staphylococcus aureus, Pseudomonas aeruginosa and Respiratory Syncitial Virus (RSV) that among other receptors can strongly activate TLR2, TLR4 and TLR3, respectively. Experiment Overall Design: All conditions were done in triplicates except for Staphylococcus aureus, were two replicates were done. As a control, unstimulated BEAS-2B were used. Altogether 11 arrays were hybridized.

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