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E-GEOD-77576

ABSTRACT: Transcription profiling by array of tbx5, nkx2-5 or both transcription factor knockouts during early heart formation in mouse

OTHER RELATED OMICS DATASETS IN: PRJNA310768PRJNA310968PRJNA310767

PROVIDER: E-GEOD-77576 | ExpressionAtlas | 2016-04-07

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-77576-analysis-methods.tsv	Tabular
	E-GEOD-77576-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-77576-configuration.xml	Xml
	E-GEOD-77576-percentile-ranks.tsv	Tabular
	E-GEOD-77576.condensed-sdrf.tsv	Tabular

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Complex Interdependence Regulates Heterotypic Transcription Factor Distribution and Coordinates Cardiogenesis [microarray]

Project description:In the developing heart, heterotypic transcription factors (TFs) interactions, such as between the T-box TF TBX5 and the homeodomain TF NKX2-5 have been proposed as a mechanism for human congenital heart disease. In order to study the role of each TF during heart formation, embryonic stem (ES) cell-derived embryos were generated from KO ES cells for Tbx5, Nkx2-5 or both TFs. We used microarrays to identify changes in the gene expression due to the lack of Tbx5, Nkx2-5 or both TFs during early heart formation. WT, Nkx2-5KO (NKO), Tbx5KO (TKO) and Tbx5KO;Nkx2-5KO (DKO) E8.75 mouse hearts were microdissected for RNA extraction and hybridization on Affymetrix microarrays.

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