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E-GEOD-77720

ABSTRACT: Transcription profiling by high throughput sequencing in brain and spinal cord of C9orf72 BAC transgenic mice

OTHER RELATED OMICS DATASETS IN: PRJNA311306

PROVIDER: E-GEOD-77720 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-77720-analysis-methods.tsv	Tabular
	E-GEOD-77720-analytics.tsv	Tabular
	E-GEOD-77720-analytics.tsv.undecorated	Tabular
	E-GEOD-77720-analytics.tsv.undecorated.unrounded	Tabular
	E-GEOD-77720-analytics.tsv.unrounded	Tabular

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C9orf72 BAC Transgenic Mice Display Typical Pathologic Features of ALS/FTD

Project description:Noncoding expansions of a hexanucleotide repeat (GGGGCC) in the C9orf72 gene are the most common cause of familial amyotrophic lateral sclerosis and frontotemporal dementia. Here we report transgenic mice carrying a bacterial artificial chromosome (BAC) containing the full human C9orf72 gene with either a normal allele (15 repeats) or disease-associated expansion (~100â??1,000 repeats; C9-BACexp). C9-BACexp mice displayed pathologic features seen in C9orf72 expansion patients, including widespread RNA foci and repeat-associated non-ATG (RAN) translated dipeptides, which were suppressed by antisense oligonucleotides targeting human C9orf72. Nucleolin distribution was altered, supporting that either C9orf72 transcripts or RAN dipeptides promote nucleolar dysfunction. Despite early and widespread production of RNA foci and RAN dipeptides in C9-BACexp mice, behavioral abnormalities and neurodegeneration were not observed even at advanced ages, supporting the hypothesis that RNA foci and RAN dipeptides occur presymptomatically and are not sufficient to drive neurodegeneration in mice at levels seen in patients. To compare the RNA Seq profiles from the cortex and spinal cord of transgenic mice expressing unexpanded human C9orf72 (F08, n=4), expanded human C9orf72 (F112, n=3/4), and nontransgenic controls (n=4).

2016-02-10 | E-GEOD-77720 | biostudies-arrayexpress

Targeted degradation of sense and antisense C9orf72 RNA foci as therapy for amyotrophic lateral sclerosis and frontotemporal dementia (strand specific RNA-seq)

Project description:Purpose: The purpose of this experiment is to identify expression changes after ASO-dependent depletion of mouse C9orf72 in the spinal cord of wild-type C57Bl/6 female mice. Methods: Strand specific RNA-seq was performed using RNAs extracted from spinal cord of C57Bl/6 mice two weeks after intracerebroventricular stereotactic injection of saline (n=3), a control ASO (n=3) or an ASO targeting mouse C9orf72 (n=3). C9orf72 RNA levels were reduced to approximately 30% of control levels in spinal cords from mice treated with the C9orf72 ASO. Results: Statistical comparison of RPKM values between RNAs from C9orf72 and control ASO treated animals or C9orf72 and saline treated samples revealed that only 12 genes were consistently upregulated (defined by P<0.05 adjusted for multiple testing) and 12 genes including C9orf72 were downregulated (defined by P<0.05 adjusted for multiple testing). Conclusions: Only few RNA expression changes were identified in the spinal cord following reduction of C9orf72. Use of strand specific RNA-seq to test the consequences of C9orf72 loss of function in mouse spinal cord.

2013-11-12 | E-GEOD-51685 | biostudies-arrayexpress

E-GEOD-77240

Project description:Transcription profiling by high throughput sequencing of a grape transgenic line overexpressing VvERF045

2017-02-20 | E-GEOD-77240 | ExpressionAtlas

E-MTAB-4786

Project description:Transcription profiling by high throughput sequencing of CD8 single positive thymocytes from F5 Rag1KO TCR transgenic mice stimulated with tumor necrosis factor

2019-05-14 | E-MTAB-4786 | ExpressionAtlas

E-GEOD-75983

Project description:Transcription profiling by high throughput sequencing of Arabidopsis pIAA26::IAA26-P108H-GFP transgenic plants

2017-02-20 | E-GEOD-75983 | ExpressionAtlas

E-GEOD-42912

Project description:Transcription profiling by high throughput sequencing of midbrain Nurr1 knockout mutant mice

2019-05-14 | E-GEOD-42912 | ExpressionAtlas

E-GEOD-78701

Project description:Transcription profiling by high throughput sequencing of uterine-specific Tsc2-null mice

2019-05-14 | E-GEOD-78701 | ExpressionAtlas

E-GEOD-52202

Project description:Transcription profiling by high throughput sequencing of iPSC derived motor neuron cultures from C9ORF72 carriers

2025-01-24 | E-GEOD-52202 | ExpressionAtlas

E-GEOD-51742

Project description:Transcription profiling by high throughput sequencing of maize transgenic plants expressing a truncated AtHB17 protein

2019-05-14 | E-GEOD-51742 | ExpressionAtlas

E-MTAB-4811

Project description:Transcription profiling by high throughput sequencing of Zika virus-infected human brain organoids

2025-01-23 | E-MTAB-4811 | ExpressionAtlas

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