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E-GEOD-98873

ABSTRACT: RNASeq analysis of Vitis vinifera (cv. Sauvignon blanc) grape berries exposed to elevated light

PROVIDER: E-GEOD-98873 | ExpressionAtlas | 2020-04-03

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-98873-analysis-methods.tsv	Tabular
	E-GEOD-98873-analytics.tsv	Tabular
	E-GEOD-98873-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-98873-configuration.xml	Xml
	E-GEOD-98873-g2_g1-mvaPlot.png	Other

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Vitis vinifera

Project description:RNASeq analysis of Vitis vinifera (cv. Sauvignon blanc) grape berries exposed to elevated light

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Dissecting the Variations of Transcriptome in Grape Berries Grown under Double Cropping System

Project description:A double cropping system has been commercially adopted in subtropical regions in southern China, where there is abundant sunshine and heat resources. In this viticulture system, the first growing season normally starts as a summer cropping cycle; then, the vine is pruned and forced by hydrogen cyanamide, resulting in a second crop in January of the next year. Due to climate differences between the two growing seasons,the transcriptome varies greatly between summer- and winter- grape berries. In this study, we conducted RNA-seq for two table grape varieties (Vitis vinifera cv. Victoria and V. vinifera cv. Muscat Superior) at three developmental stages (pre-veraison, post-veraison and full maturity).The transcriptome between summer- and winter- grape berries were compared.

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Project description:Transcriptional changes occurring in grape berries (Vitis vinefera L. cv Cabernet Sauvignon) cultured in vitro with high (468 mM) and low (58 mM) concentrations of glucose in the culture medium was verified. Gene expression profiling was done using the Nimblegen whole genome array with 3 biological replicates.

2014-07-16 | E-MTAB-1634 | biostudies-arrayexpress

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Project description:We used Affymetrix microarray analyses of thirty-two individual Vitis vinifera cv. Cabernet Sauvignon berries sampled from two clusters at fifty-percent ripening initiation. By delineating four developmental stages of ripening initiation, we demonstrate that color is a statistically significant indicator of transcriptional state during ripening initiation. We report on clustered gene expression patterns which were mined for genes annotated with signal transduction functions in order to advance regulatory network modeling of ripening initiation in grape berries. We also demonstrated that gene expression does not differ statistically significantly at a global level in berries sampled from different plants or different positions in a cluster. Experiment Overall Design: Individual grape berries were selected at 4 developmental stages along ripening initiation for RNA extraction and hybridization to Affymetrix Vitis Genome GeneChips. One goal of this study was to determine the association between the global transcriptome state and phenotypic variables frequently used in berry staging such as color and firmness, so we defined the four time-points (development series) by color/firmness combinations: green hard (GH), green soft (GS), pink soft (PS) and red soft (RS). Individual berries representing each stage were collected from either the distal or the proximal position of one cluster one each of two plants used for experiments.

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Project description:Methods:transcriptomes of the different development stages of Vitis vinifera cv. Cabernet Sauvignon and Vitis quinquangularis accession Danfeng-2 were analyzed using Illumina Hiseq 2500. The sequence reads that passed quality filters were analyzed: TopHat followed by Cufflinks. mRNA profiles of different development stages of Vitis vinifera cv. Cabernet Sauvignon and Vitis quinquangularis accession Danfeng-2 were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500.

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Grape skin transcriptome to reveal genes related with resveratrol accumulation

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Light-induced variation in phenolic compounds in Cabernet Sauvignon grapes (Vitis vinifera L.) involves extensive transcriptome reprogramming of biosynthetic enzymes, transcription factors, and phytohormonal regulators

Project description:Light environments have long been known to influence grape (Vitis vinifera L.) berry development and biosynthesis of phenolic compounds, and ultimately affect wine quality. Here, the accumulation and compositional changes of hydroxycinnamic acids (HCAs) and flavonoids, as well as global gene expression were analyzed in Cabernet Sauvignon grape berries under sunlight exposure treatments at different phenological stages. Sunlight exposure did not consistently affect the accumulation of berry skin flavan-3-ol or anthocyanin among different seasons due to climatic variations, but increased HCA content significantly at véraison and harvest, and enhanced flavonol accumulation dramatically with its timing and severity degree trend. As in sunlight exposed berries, a highly significant correlation was observed between the expression of genes coding phenylalanine ammonia-lyase, 4-coumarate: CoA ligase, flavanone 3-hydroxylase and flavonol synthase family members and corresponding metabolite accumulation in the phenolic biosynthesis pathway, which may positively or negatively be regulated by MYB, bHLH, WRKY, AP2/EREBP, C2C2, NAC, and C2H2 transcription factors (TFs). Furthermore, some candidate genes required for auxin, ethylene and abscisic acid signal transductions were also identified which are probably involved in berry development and flavonoid biosynthesis in response to enhanced sunlight irradiation. Taken together, this study provides a valuable overview of the light-induced phenolic metabolism and transcriptome changes, especially the dynamic responses of TFs and signaling components of phytohormones, and contributes to the further understanding of sunlight-responsive phenolic biosynthesis regulation in grape berries.

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Auto-grafting in grapevine 3 and 28 days after grafting

Project description:The analysis of the genes differentially expressed in the rootstock and the callus 3 and 28 d after grafting in grapevine (Vitis vinifera cv Cabernet Sauvignon) auto-grafts.

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