Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-MEXP-2652

ABSTRACT: Transcription profiling by array of Drosophila salivary glands upon NSL3, MCRS2 and MBDR2 RNAi mediated depletion

OTHER RELATED OMICS DATASETS IN: PRJNA133827

PROVIDER: E-MEXP-2652 | ExpressionAtlas | 2015-10-22

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-MEXP-2652-analysis-methods.tsv	Tabular
	E-MEXP-2652-atlasExperimentSummary.Rdata	Rdata
	E-MEXP-2652-configuration.xml	Xml
	E-MEXP-2652-percentile-ranks.tsv	Tabular
	E-MEXP-2652.condensed-sdrf.tsv	Tabular

Items per page:

1 - 5 of 83

Similar Datasets

RNAi profiling by array of Drosophila salivary glands upon NSL3, MCRS2 and MBDR2 RNAi mediated depletion

Project description:Expression profiling of NSL3, MCRS2 and MBDR2 RNAi-mediated depletion in Drosophila salivary glands. This experiment is related to experiment E-MTAB-214

2010-06-25 | E-MEXP-2652 | biostudies-arrayexpress

sage gene RNAi experiment

Project description:Comparisons between the salivary glands upon the RNAi of sage gene vs. the control. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development Keywords: other

2003-07-23 | GSE283 | GEO

sage gene RNAi experiment

2010-06-05 | E-GEOD-283 | biostudies-arrayexpress

Phosphatidylserine metabolism affects mitochondrial homeostasis in Drosophila

Project description:In Drosophila, insufficient biosynthesis of phosphatidylserine induced significant mitochondrial defects.Proteomic analysis of samples from drosophila salivary glands, we compared three genotypes, ppl-gal4 drived RNAi against w, pss and pss plus bbc.

2023-10-23 | PXD046310 |

Drosophila ROW RNAi gene expression profiling

Project description:Heterochromatin-protein 1 (HP1) is a functionally diverse family of proteins. In particular, Drosophila dHP1c forms a complex with the transcription factors WOC and ROW (dHP1EU) that localizes at euchromatin and regulates gene expression. We used microarrays to analyse the changes in gene expression after row depletion by RNAi. For expression profiling analyses, total RNAs were prepared from control S2 cells and upon RNAi-mediated depletion of ROW, converted to cDNA and hybridized to Drosophila Genome 2.0 GeneChip (Affymetrix).

2015-05-01 | E-GEOD-49103 | biostudies-arrayexpress

Dissecting tissue-specific transcriptional response to 20E in Drosophila by E23 expression

Project description:Transcriptional activation by 20-hydroxyecdysone (20E) in Drosophila provides an excellent model for studying tissue-specific response to steroids. An increase in 20E titer controls degradation of larval and proliferation of adult tissues during metamorphosis. To study 20E-dependent transcription, we used the natural system for controlling the 20E titer – E23 membrane transporter, which exports 20E from the cell. We artificially expressed the E23 in tissues to suppress the first wave of 20E-inducible transcription at metamorphosis. Expression of E23 revealed a plethora of 20H dependent genes in salivary glands, but mildly affected the transcription in brain/eye-antennal discs. We described the mechanisms controlling the transcriptional activation by 20E in Drosophila salivary glands. 20E depletion caused a decreased binding of Pol II and TFIID subunit, TBP, to target promoters, demonstrating 20E role in the stimulation of transcription initiation. At the target loci 20E depletion resulted in a malfunctioning of sites co-bound with EcR and CBP/Nejire and enriched with H3K27Ac histone mark inherent to active enhancers. At these sites, 20E titer was found to control chromatin accessibility and acetylation. We presume that the activity of these ‘active’ ecdysone-sensitive elements – (ESEs) is responsible for an active status of 20E targets in the salivary glands of wandering larvae.

2025-03-31 | GSE260939 | GEO

Developmental Control of Gene Copy Number by Repression of Replication Initiation and Fork Progression

Project description:This SuperSeries is composed of the following subset Series: GSE31895: ChIP with anti-orc2 antibody to identify regions of orc binding in third instar salivary glands of WT and SuUR mutant Drosophila GSE31896: RNAPolII ChIP to find differences between third instar salivary glands of WT and SuUR GSE31897: ChIP with anti-H3K27me3 to compare binding in salivary glands of WT and SuUR Drosophila GSE31898: CGH to ascertain levels of gDNA in third instar salivary glands of various mutant Drosophila GSE31899: ChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing GSE33017: Expression profile of third instar larval salivary gland tissue Refer to individual Series

2011-10-17 | E-GEOD-31900 | biostudies-arrayexpress

Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1 [Affymetrix Expression]

Project description:Indicated cells were subjected to RNAi against linker histone H1, Nautilus (control), or GFP (control). RNA was isolated and subjected to Affymetrix GeneChIP Drosophila Genome 2.0 arrays RNA was compared from cells treated with RNAi against control (Nautilus in the case of salivary glands or GFP in the case of Kc cells) and linker histone H1. Kc cells were treated once with RNAi and RNA was collected 6 days later.

2013-04-22 | E-GEOD-44398 | biostudies-arrayexpress

ChIP with anti-H3K27me3 to compare binding in salivary glands of WT and SuUR Drosophila

Project description:ChIP was performed to identify regions of gDNA bound by H3K27me3 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that H3K27me3 binds differently in under-replicated in SuUR mutant third instar salivary glands.

2011-10-18 | GSE31897 | GEO

RNAPolII ChIP to find differences between third instar salivary glands of WT and SuUR

Project description:ChIP was performed to identify regions of gDNA bound by RNA polymerase II in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that RNAPolII does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands. Comparison of RNAPolII ChIP from third instar salivary glands relative to control IgG pulldown for two different Drosophila strains

2011-10-17 | E-GEOD-31896 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data