Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sage gene RNAi experiment

ABSTRACT: Comparisons between the salivary glands upon the RNAi of sage gene vs. the control. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Tong-Ruei Li

PROVIDER: E-GEOD-283 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Tissue-specific gene expression and ecdysone-regulated genomic networks in Drosophila.

Li Tong-Ruei TR White Kevin P KP

Developmental cell 20030701 1

During insect metamorphosis, each tissue displays a unique physiological and morphological response to the steroid hormone 20-hydroxyecdysone (ecdysone). We assayed gene expression in five tissues during metamorphosis onset. Larval-specific tissues display major changes in genome-wide expression profiles, whereas tissues that survive into adulthood display few changes. In one larval tissue, the salivary gland, we used a computational approach to identify a regulatory motif and a cognate transcri ...[more]

PMID: 12852852

Similar Datasets

New targets of Drosophila adenosine deaminase acting on RNA (dADAR)

Project description:The aim of this experiment is to identify new targets of Drosophila adenosine deaminase acting on RNA (dADAR) by enriching Inosine-containing mRNA (I-mRNA) from wild type (C-S) and dADAR mutant flies and then hybridization with Drosophila cDNA arrays.

2010-06-05 | E-GEOD-529 | biostudies-arrayexpress

Heterogous hybridization experiment

Project description:Fish and Chips: Expression Profiling in Non-traditional Model Systems Using a Cichlid Fish cDNA Microarray This series represents the 26 arrays that went into Renn et al 2004 (submitted January 16th), and one additional hybridization (GSM15240) that was not included in the publication analysis for statistical reasons.

2010-06-09 | E-GEOD-975 | biostudies-arrayexpress

S. cerevisiae mutant with a constitutively activated Ras/cAMP pathway

Project description:Comparison of the transcriptomes of Saccharomyces cerevisiae wild type FY23 and a PDE2 deletion mutant DJ28. Keywords = PDE2 Keywords = Ras/cAMP pathway

2010-06-05 | E-GEOD-600 | biostudies-arrayexpress

Life cycle of Drosophila melanogaster

Project description:Molecular genetic studies of Drosophila melanogaster have led to profound advances in understanding the regulation of development. Here we report gene expression patterns for nearly one-third of all Drosophila genes during a complete time course of development. Mutations that eliminate eye or germline tissue were used to further analyze tissue-specific gene expression programs. These studies define major characteristics of the transcriptional programs that underlie the life cycle, compare development in males and females, and show that large-scale gene expression data collected from whole animals can be used to identify genes expressed in particular tissues and organs or genes involved in specific biological and biochemical processes.

2003-01-05 | E-GEOD-94 | biostudies-arrayexpress

MES-4, an autosome-associated HMT that participates in silencing the X chromosomes in the C. elegans germ line

Project description:Microarray-based expression profiling of dissected gonads from mes-4 mutants reveals that MES-4 is required to repress expression of a set of X-linked genes. We isolated dissected gonads from mes-4(bn85) mutant adults and compared to dissected gonads from wild type (N2 animals. RNA was linearly amplified prior to labeling for all genotypes. Comparison was done in quadruplicate on independently grown and isolated animals.

2010-07-01 | E-GEOD-5454 | biostudies-arrayexpress

Promotion of oogenesis and embryogenesis in the C. elegans gonad by EFL-1/DPL-1(E2F)does not require LIN-35(pRB)

Project description:Microarray-based expression profiling of dissected gonads from efl-1, dpl-1 and lin-35 mutants reveals that EFL-1 and DPL-1 promote expression of an extensively overlapping set of target genes, consistent with the expectation that these two proteins function as a heterodimer. Regulatory regions upstream of many of these target genes have a canonical E2F binding site, suggesting that their regulation by EFL-1/DPL-1 is direct. Many EFL-1/DPL-1 responsive genes encode proteins required for oogenesis and early embryogenesis, rather than cell cycle components. By contrast, LIN-35 appears to function primarily as a repressor of gene expression in the germ line, and the genes that it acts on are for the most part distinct from those regulated by EFL-1 and/or DPL-1 We isolated dissected gonads from dpl-1, efl-1 and lin-35 mutant adults and compared each to control dissected gonads. RNA was linearly amplified prior to labeling for all genotypes. Each comparison was done in triplicate (dpl-1 and lin-35) or in quadruplicate (efl-1) on independently grown and isolated animals.

2010-07-01 | E-GEOD-5071 | biostudies-arrayexpress

Porcine liver

Project description:Fasting, fed, and Clofibric Acid comparisons

2003-11-14 | E-GEOD-714 | biostudies-arrayexpress

Cross species comparison of expression patterns

Project description:Comparison of expression in liver samples of human, chimp, orang and rhesus, by using a novel multi-species cDNA array

2010-06-10 | E-GEOD-2009 | biostudies-arrayexpress

CX43 KO vs WT cortical astrocytes

Project description:RNA samples extracted from astrocytes cultured from wild type and Cx43 null neonatal mice were dye labeled and individually co-hybridized with a reference of labeled cDNAs pooled from a variety of tissues on eight gene arrays containing 8975 mouse DNA sequences

2010-06-05 | E-GEOD-580 | biostudies-arrayexpress

DEPS-1 promotes P granule assembly and RNA interference in C. elegans germ cells

Project description:P granules are germ-cell-specific cytoplasmic structures containing RNA and protein and required for proper germ cell development in C. elegans. A new P-granule-associated protein, DEPS-1, whose loss disrupts P granule structure and function was analysed in this study. Genome wide analysis of gene expression in deps-1 mutant germ lines identified additional targets of DEPS-1 regulation, many of which are also regulated by the RNAi factor RDE-3. Our studies suggest that DEPS-1 is a key component of the P granule assembly pathway and that its roles include promoting accumulation of some mRNAs, such as glh-1 and rde-4, and reducing accumulation of other mRNAs, perhaps by collaborating with RDE-3 to generate endogenous short interfering RNAs (endo-siRNAs). We isolated dissected gonads from deps-1 mutant adults and compared each to wild type dissected gonads. RNA was linearly amplified prior to labeling for all genotypes. The comparisons were done in quadruplicate on independently grown and isolated animals.

2010-05-26 | E-GEOD-9993 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data