Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-MIMR-461

ABSTRACT: Transcription profiling by array of mouse embryonic stem cells expressing the transcription factor NGN3 for 12 hours, 24 hours and 48 hours

PROVIDER: E-MIMR-461 | ExpressionAtlas | 2014-07-03

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-MIMR-461-analysis-methods.tsv	Tabular
	E-MIMR-461-atlasExperimentSummary.Rdata	Rdata
	E-MIMR-461-configuration.xml	Xml
	E-MIMR-461-percentile-ranks.tsv	Tabular
	E-MIMR-461.condensed-sdrf.tsv	Tabular

Items per page:

1 - 5 of 95

Similar Datasets

R1 Embryonic Stem Cell and Embryoid Body Time Course

Project description:An 11-point time course study comparing R1 embryonic stem cells versus embryoid bodies. Time course 0 hours, 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, 48 hours, 4 days, 7 days, 9 days, and 14 days. Keywords: other

2005-07-22 | GSE2972 | GEO

V6.5 Embryonic Stem Cell and Embryoid Body Time Course

Project description:An 11-point time course study comparing V6.5 embryonic stem cells versus embryoid bodies. Time course 0 hours, 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, 48 hours, 4 days, 7 days, 9 days, and 14 days. Keywords: other

2005-09-01 | GSE3231 | GEO

Transcription profiling by array of mouse embryonic stem cells expressing the transcription factor NGN3 for 12 hours, 24 hours and 48 hours

Project description:A mouse embryonic stem cell line was generated which stably expressed the ngn3 transcription factor under the control of the Tet-On inducible system using knock-ins in the ROSA26 and the HPRT loci. The undifferentiated mouse embryonic stem cells were then differentiated into Embryoid Bodies in suspension culture and were either treated with Doxycycline to induce NGN3 expression or left untreated as a contol. Cells were harvested at 12 hours, 24 hours and 48 hours.

2007-09-01 | E-MIMR-461 | biostudies-arrayexpress

Analysis of transposable element expression in mouse ESCs with and without Ncor1, and a time course RNA-seq of mouse ESC differentiation to EpiLCs

Project description:Mouse embryonic stem cells (ESCs) were differentiated to Epiblast-like cells (EpiLCs) according to the protocol in Hayashi et al., 2011. Samples were taken at 0, 12, 24, 36 and 48 hours. Wildtype and Ncor2 knockout ESCs were also sequenced to measure gene expression.

2020-07-22 | GSE137627 | GEO

Metabolic enhancement of developmental pausing (embryos)

Project description:Embryonic stem (ES) cells and embryos reversibly pause via chemical mTOR inhibition. In this study, we investigate the tissue-specific response to mTORi-induced pausing in ES and trophoblast stem (TS) cells. To resolve the sequential rewiring of the proteome, we conducted a time-series proteomics experiment at 1, 3, 6, 12, 24, and 48 hours upon induction of pausing, and at 1, 3, 6, 12, 24, and 48 hours upon release of pausing in ES and TS cells. We find that ES, but not TS cells pause reversibly. To optimise developmental pausing conditions, we reasoned that by understanding the difference in pausing response of ES and TS cells, we could identify which pathways are essential for pausing. We found that KEGG pathways related to amino acid degradation, fatty acid degradation, and DNA repair are upregulated in ES cells, but downregulated in TS cells during entry into pausing. Moreover, by targeted metabolomics, we found a depletion of short chain carnitines in the paused ES cells. To extend the length of developmental pausing, we supplemented paused embryos with L-carnitine. The L-carnitine supplementation facilitates lipid usage and prolongs the pausing length by 19 days through the establishment of a more dormant state.

2023-11-06 | PXD041325 | Pride

Metabolic enhancement of developmental pausing (TS cells)

2023-11-06 | PXD033798 | Pride

Metabolic enhancement of developmental pausing (ES dataset)

2023-11-06 | PXD033750 | Pride

Gene expression data following Neurogenin 1 expression in mouse embryonic stem cells

2013-01-15 | GSE42883 | GEO

Gene expression data following Neurogenin 1 expression in mouse embryonic stem cells

Project description:To study the early gene expression events involved in neurogenesis, we expressed the bHLH neural determination factor neurogenin 1 in mouse embryonic stem cells. We used microarray to examine the program of gene expression at 24, 48, 72 hours of transgene expression. Mouse embryonic stem cells were engineered to express neurogenin 1 using a tet-sensitive expression system. RNAs from uninduced and induced cells were isolated at 24, 48, and 72 hours of differentiation and subjected to microarray analysis.

2013-01-15 | E-GEOD-42883 | biostudies-arrayexpress

Synechococcus elongatus PCC7942 proteome's cyclic rhythm by LC-MSMS

Project description:To uncover the variation in protein abundances during 48 hours under light and dark cycles (12:12-hours), we used quantitative proteomics, with TMT 6-plex isobaric labeling. We queried the S. elongatus proteome at ten different time points spanning a single 24 hour period, leading to 20 time points over the full 48 hour period.

2014-04-02 | PXD000510 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data