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E-MTAB-2277

ABSTRACT: RNA-seq of coding RNA in human SH-SY5Y cells with shRNA knockdown of RNA-binding protein Unkempt (Unk)

PROVIDER: E-MTAB-2277 | ExpressionAtlas | 2025-01-23

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-2277-analysis-methods.tsv	Tabular
	E-MTAB-2277-analytics.tsv	Tabular
	E-MTAB-2277-analytics.tsv.undecorated	Tabular
	E-MTAB-2277-analytics.tsv.undecorated.unrounded	Tabular
	E-MTAB-2277-analytics.tsv.unrounded	Tabular

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Project description:iCLIP experiments tomap the RNA binding sites of the RNA-binding protein Unkempt across the transcriptome in SH-SY5Y cells, HeLa cells with ectopic Unk expression and mouse E15 embryonic brain samples. Expression of Unk is normally largely restricted to the nervous system. We therefore mapped the binding sites in human SH-SY5Y and mouse E15 brain to detect its physiological binding sites (in SH-SY5Y, we also performed the RNAseq experiment upon Unk knockdown). HeLa cells on the other hand normally don't express Unk, but convert to neuron-like shape when the protein is ectopically expressed. So, here we hoped to identify those binding events (and hence target transcripts) that are critical for this morphological transformation.

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