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E-PROT-73

ABSTRACT: NCI60 proteome by PCT-SWATH - Quantitative Proteome Landscape of the NCI-60 Cancer Cell Lines

PROVIDER: E-PROT-73 | ExpressionAtlas | 2022-01-26

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-PROT-73-analysis-methods.tsv	Tabular
	E-PROT-73-apply_fixes_zooma_mappings.done	Other
	E-PROT-73-configuration.xml	Xml
	E-PROT-73-factors.xml	Xml
	E-PROT-73-raw-counts_MappedToGeneID.txt	Txt

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Proteomic profiling of NCI60 cell lines from Cancer Cell Line Encyclopedia

Project description:This ArrayExpress record contains meta-data and results of quantitative analysis of cell lines from the NCI-60 panel using pressure cycling technology (PCT) and SWATH-mass spectrometry. Each cell line was analyzed in duplicate. Raw data files are available at the EMBL-EBI protemics data archive (PRIDE) at accession PXD003539 (http://www.ebi.ac.uk/pride/archive/projects/PXD003539). Since the record here does not include the raw data files and hence there is no need to explicitly link individual replicate to a raw file, each sample is only listed once in the ArrayExpress samples table for clarity.

2017-01-27 | E-PROT-2 | biostudies-arrayexpress

NCI60 proteome by PCT-SWATH - Quantitative Proteome Landscape of the NCI-60 Cancer Cell Lines

Project description:We obtained quantitative analysis of the NCI-60 cell panels using pressure cycling technology (PCT) and SWATH-MS. Each cell was analyzed in duplicate. The data were analyzed using a cell line SWATH assay library and OpenSWATH, followed by SWATH-expert refinement.

2020-06-01 | PXD003539 | Pride

NCI60 proteome by PCT-SWATH - Quantitative Proteome Landscape of the NCI-60 Cancer Cell Lines

2020-07-29 | MSV000085848 | MassIVE

E-PROT-70

Project description:PCP39: prostate cancer proteome for 39 patients by PCT-SWATH

2022-01-26 | E-PROT-70 | ExpressionAtlas

E-PROT-25

Project description:Global Proteome Analysis of the NCI-60 Cell Line Panel

2021-05-25 | E-PROT-25 | ExpressionAtlas

Comparison of protein expression profiles in NCI-60 cell panel containing nine difererent cancer tissue types.

Project description:Because most potential molecular markers and targets are proteins, proteomic profiling is expected to yield more direct answers to functional and pharmacological questions than does transcriptional profiling. To aid in such studies, we have developed a protocol for making reverse-phase protein lysate microarrays with larger numbers of spots than previously feasible. Our first application of these arrays was to profiling of the 60 human cancer cell lines (NCI-60) used by the National Cancer Institute to screen compounds for anticancer activity. Each glass slide microarray included 648 lysate spots representing the NCI-60 cell lines plus controls, each at 10 two-fold serial dilutions to provide a wide dynamic range. Mouse monoclonal antibodies and the catalyzed signal amplification system were used for immunoquantitation. The signal levels from the >30,000 data points for our first 52 antibodies were analyzed by using P-SCAN and a quantitative dose interpolation method. Clustered image maps revealed biologically interpretable patterns of protein expression. Among the principal early findings from these arrays were two promising pathological markers for distinguishing colon from ovarian adenocarcinomas. When we compared the patterns of protein expression with those we had obtained for the same genes at the mRNA level by using both cDNA and oligonucleotide arrays, a striking regularity appeared: cell-structure-related proteins almost invariably showed a high correlation between mRNA and protein levels across the NCI-60 cell lines, whereas non-cell-structure-related proteins showed poor correlation. http://discover.nci.nih.gov/host/2003_profiling_abstract.jsp Keywords: Cancer cell line comparison.

2007-02-01 | GSE5501 | GEO

E-PROT-67

Project description:113 DLBCL SWATH maps by PCT-SWATH

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DDA files of human cancer cells for the NCI-60 SWATH project PXD003539

Project description:This is the raw files for the DDA library used for intepreting the NCI-60 SWATH project PXD003539

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NCI-60 Cancer Cell Line

Project description:NCI-60 cancer cell lines were profiled with their genome-wide gene expression patterns using Affymetrix HG-U133A chips. Keywords: NCI-60 cancer cell line expression profiling

2009-08-26 | GSE5846 | GEO

E-PROT-69

Project description:76 human liver tissue proteomes by PCT-SWATH

2022-01-26 | E-PROT-69 | ExpressionAtlas