Dataset Information

Kim2011 - the genome scale reconstruction of the Vibrio vulnificus metabolic network - VvuMBEL943

ABSTRACT: This is a model of the genome scale reconstruction of the Vibrio vulnificus metabolic network, VvuMBEL943, described in the article: Integrative genome-scale metabolic analysis of Vibrio vulnificus for drug targeting and discovery Hyun Uk Kim, Soo Young Kim, Haeyoung Jeong, Tae Yong Kim, Jae Jong Kim, Hyon E Choy, Kyu Yang Yi, Joon Haeng Rhee, and Sang Yup Lee. Molecular Systems Biology 7:460 Jan 2011 doi: 10.1038/msb.2010.115 Abstract: Although the genomes of many microbial pathogens have been studied to help identify effective drug targets and novel drugs, such efforts have not yet reached full fruition. In this study, we report a systems biological approach that efficiently utilizes genomic information for drug targeting and discovery, and apply this approach to the opportunistic pathogen Vibrio vulnificus CMCP6. First, we partially re-sequenced and fully re-annotated the V. vulnificus CMCP6 genome, and accordingly reconstructed its genome-scale metabolic network, VvuMBEL943. The validated network model was employed to systematically predict drug targets using the concept of metabolite essentiality, along with additional filtering criteria. Target genes encoding enzymes that interact with the five essential metabolites finally selected were experimentally validated. These five essential metabolites are critical to the survival of the cell, and hence were used to guide the cost-effective selection of chemical analogs, which were then screened for antimicrobial activity in a whole-cell assay. This approach is expected to help fill the existing gap between genomics and drug discovery. This metabolic network model has been thoroughly validated by the authors. VvuMBEL943 is a stoichiometric model that contains the metabolic information of the microbial pathogen, Vibrio vulnificus CMCP6, at genome-scale. The SBML version was generated by Hyun Uk Kim using MetaFluxNet. This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not.. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.

SUBMITTER: Hyun Uk Kim

PROVIDER: BIOMD0000001064 | BioModels | 2024-09-02

REPOSITORIES: BioModels

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			Action	DRS
	BIOMD0000001064?filename=Kim2011_VvuMBEL943-biopax2.owl	Owl
	BIOMD0000001064?filename=Kim2011_VvuMBEL943-biopax3.owl	Owl
	BIOMD0000001064?filename=Kim2011_VvuMBEL943.xml	Xml
	BIOMD0000001064?filename=MemoteReport-MODEL1011300000.html	Other
	BIOMD0000001064?filename=VvuMBEL943_miniFROG.xlsx	Xlsx

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Integrative genome-scale metabolic analysis of Vibrio vulnificus for drug targeting and discovery.

Kim Hyun Uk HU Kim Soo Young SY Jeong Haeyoung H Kim Tae Yong TY Kim Jae Jong JJ Choy Hyon E HE Yi Kyu Yang KY Rhee Joon Haeng JH Lee Sang Yup SY

Molecular systems biology 20110101

Although the genomes of many microbial pathogens have been studied to help identify effective drug targets and novel drugs, such efforts have not yet reached full fruition. In this study, we report a systems biological approach that efficiently utilizes genomic information for drug targeting and discovery, and apply this approach to the opportunistic pathogen Vibrio vulnificus CMCP6. First, we partially re-sequenced and fully re-annotated the V. vulnificus CMCP6 genome, and accordingly reconstru ...[more]

PMID: 21245845

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