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Transcription profiling of rat heart biopsies with and without RNA amplification

ABSTRACT:

ORGANISM(S): Rattus norvegicus

SUBMITTER: Rachel van Haaften

PROVIDER: E-AFMX-10 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Biologically relevant effects of mRNA amplification on gene expression profiles.

van Haaften Rachel I M RI Schroen Blanche B Janssen Ben J A BJ van Erk Arie A Debets Jacques J M JJ Smeets Hubert J M HJ Smits Jos F M JF van den Wijngaard Arthur A Pinto Yigal M YM Evelo Chris T A CT

BMC bioinformatics 20060411

<h4>Background</h4>Gene expression microarray technology permits the analysis of global gene expression profiles. The amount of sample needed limits the use of small excision biopsies and/or needle biopsies from human or animal tissues. Linear amplification techniques have been developed to increase the amount of sample derived cDNA. These amplified samples can be hybridised on microarrays. However, little information is available whether microarrays based on amplified and unamplified material y ...[more]

PMID: 16608515

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