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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Mycobacterium tuberculosis treated with benzothiazinones which kill M. tuberculosis by blocking arabinan synthesis

ABSTRACT: Data is also available from BuG@Sbase

INSTRUMENT(S): Nanodrop N1000 spectrophotometer

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER:

PROVIDER: E-BUGS-80 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Benzothiazinones kill Mycobacterium tuberculosis by blocking arabinan synthesis.

Makarov Vadim V Manina Giulia G Mikusova Katarina K Möllmann Ute U Ryabova Olga O Saint-Joanis Brigitte B Dhar Neeraj N Pasca Maria Rosalia MR Buroni Silvia S Lucarelli Anna Paola AP Milano Anna A De Rossi Edda E Belanova Martina M Bobovska Adela A Dianiskova Petronela P Kordulakova Jana J Sala Claudia C Fullam Elizabeth E Schneider Patricia P McKinney John D JD Brodin Priscille P Christophe Thierry T Waddell Simon S Butcher Philip P Albrethsen Jakob J Rosenkrands Ida I Brosch Roland R Nandi Vrinda V Bharath Sowmya S Gaonkar Sheshagiri S Shandil Radha K RK Balasubramanian Venkataraman V Balganesh Tanjore T Tyagi Sandeep S Grosset Jacques J Riccardi Giovanna G Cole Stewart T ST

Science (New York, N.Y.) 20090319 5928

New drugs are required to counter the tuberculosis (TB) pandemic. Here, we describe the synthesis and characterization of 1,3-benzothiazin-4-ones (BTZs), a new class of antimycobacterial agents that kill Mycobacterium tuberculosis in vitro, ex vivo, and in mouse models of TB. Using genetics and biochemistry, we identified the enzyme decaprenylphosphoryl-beta-d-ribose 2'-epimerase as a major BTZ target. Inhibition of this enzymatic activity abolishes the formation of decaprenylphosphoryl arabinos ...[more]

PMID: 19299584

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Transcription profiling of Mycobacterium tuberculosis infected human dendritic cells and macrophages and M. tuberculosis grown aerobically to investigate host-pathogen cross-talk

Project description:Data is also available from http://bugs.sgul.ac.uk/E-BUGS-58

2008-01-04 | E-BUGS-58 | biostudies-arrayexpress

Methionine Sulfoximine Resistance in Mycobacterium tuberculosis Is Due to a Single Nucleotide Deletion Resulting in Increased Expression of the Major Glutamine Synthetase, GlnA1.

Project description:We investigated the effect of methionine sulfoximine (MetSox), a potent inhibitor of glutamine synthetase, on Mycobacterium tuberculosis. M. tuberculosis encodes four glutamine synthetases, of which MetSox targets the type I enzyme encoded by glnA1. Trancriptional profiling revealed that glutamate synthetase (gltB) and a type II glutamine synthetase (glnA3) were induced after exposure to MetSox. In addition, we observed a high rate (10(-5)) of spontaneous resistance to MetSox. All resistant strains had a single-nucleotide deletion in the 5' region of glnA1, and Western analysis revealed that GlnA1 expression was increased in resistant as compared with sensitive strains. These data show that M. tuberculosis can respond to the effect of MetSox inhibition either by up-regulation of GlnA3 or by GlnA1. The high frequency of resistance suggests that MetSox and other compounds specifically targeting GlnA1 are not likely to become successful anti-mycobacterial agents. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-112

2011-09-06 | E-BUGS-112 | biostudies-arrayexpress

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Project description:Data is also available from http://bugs.sgul.ac.uk/E-BUGS-63

2008-09-26 | E-BUGS-63 | biostudies-arrayexpress

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Project description:Data is also available from http://bugs.sgul.ac.uk/E-BUGS-52

2008-04-02 | E-BUGS-52 | biostudies-arrayexpress

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Project description:Data is also available from <ahref=http://bugs.sgul.ac.uk/E-BUGS-81 target=_blank>BuG@Sbase</a>

2009-11-25 | E-BUGS-81 | biostudies-arrayexpress

Transcription profiling by array of Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection

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