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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Comparative genomic hybridization by array of Streptococcus pneumoniae isolates from the Mitis group to identify pneumococcal virulance genes

ABSTRACT: Data is also available from BuG@Sbase

INSTRUMENT(S): NanoDrop

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER:

PROVIDER: E-BUGS-82 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Detection of large numbers of pneumococcal virulence genes in streptococci of the mitis group.

Johnston Calum C Hinds Jason J Smith Andrew A van der Linden Mark M Van Eldere Johan J Mitchell Tim J TJ

Journal of clinical microbiology 20100602 8

Seven streptococcal isolates from the mitis group were analyzed for the presence of pneumococcal gene homologues by comparative genomic hybridization studies with microarrays based on open reading frames from the genomes of Streptococcus pneumoniae TIGR4 and R6. The diversity of pneumolysin (ply) and neuraminidase A (nanA) gene sequences was explored in more detail in a collection of 14 S. pseudopneumoniae and 29 mitis group isolates, respectively. The mitis group isolates used in the microarray ...[more]

PMID: 20519466

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Transcription profiling of Staphylococcus aureus treated with tigecycline to determine the effect of the expression of virulence factors in biofilm-associated cells

Project description:Data is also available from <ahref=http://bugs.sgul.ac.uk/E-BUGS-81 target=_blank>BuG@Sbase</a>

2009-11-25 | E-BUGS-81 | biostudies-arrayexpress

The distribution of mobile genetic elements (MGEs) in MRSA CC398 is associated with both host and country.

Project description:Methicillin-resistant Staphylococcus aureus clonal complex (CC) 398 has emerged from pigs to cause human infections in Europe and North America. We used a new 62-strain S. aureus microarray (SAM-62) to compare genomes of isolates from three geographical areas (Belgium, Denmark, and Netherlands) to understand how CC398 colonizes different mammalian hosts. The core genomes of 44 pig isolates and 32 isolates from humans did not vary. However, mobile genetic element (MGE) distribution was variable including SCCmec. Phi3 bacteriophage and human specificity genes (chp, sak, scn) were found in invasive human but not pig isolates. SaPI5 and putative ruminant specificity gene variants (vwb and scn) were common but not pig specific. Virulence and resistance gene carriage was host associated but country specific. We conclude MGE exchange is frequent in CC398 and greatest among populations in close contact. This feature may help determine epidemiological associations among isolates of the same lineage. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-120]

2012-01-21 | E-BUGS-120 | biostudies-arrayexpress

Staphylococcus aureus CC398 Clade Associated with Human-to-Human Transmission

Project description:Staphylococcus aureus clonal complex 398 (CC398) isolates colonize livestock and can spread to human contacts. Genetic analysis of isolates epidemiologically associated with human-to-human, but not livestock, transmission in multiple countries and continents identified a common clade that was negative for tet(M) and positive for bacteriophage 3. Another group of human-to-human-transmitted isolates belonged to the common livestock-associated clade but had acquired a unique φ7 bacteriophage. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-124]

2012-10-29 | E-BUGS-124 | biostudies-arrayexpress

Detection of mobile-genetic-element variation between colonizing and infecting hospital-associated methicillin-resistant Staphylococcus aureus isolates.

Project description:Whole-genome analysis by 62-strain microarray showed variation in resistance and virulence genes on mobile genetic elements (MGEs) between 40 isolates of methicillin-resistant Staphylococcus aureus (MRSA) strain CC22-SCCmecIV but also showed (i) detection of two previously unrecognized MRSA transmission events and (ii) that 7/8 patients were infected with a variant of their own colonizing isolate. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-128]

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Project description:Data is also available from http://bugs.sgul.ac.uk/E-BUGS-77

2009-10-12 | E-BUGS-77 | biostudies-arrayexpress

Comparative genomic hybridization of S. aureus to examine Genetic Diversity in CC398 Methicillin-Resistant Staphylococcus aureus of Different Geographic Origin.

Project description:Data is also available from <ahref=http://bugs.sgul.ac.uk/E-BUGS-84 target=_blank>BuG@Sbase</a>

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Transcription profiling by array of Mycobacterium tuberculosis to analyse the regulatory role of the HigA antitoxin

Project description:Data is also available from <ahref=http://bugs.sgul.ac.uk/E-BUGS-107 target=_blank>BuG@Sbase</a>

2010-07-16 | E-BUGS-107 | biostudies-arrayexpress

The minor groove-binding agent ELB-21 forms multiple interstrand and intrastrand covalent cross-links with duplex DNA and displays potent bactericidal activity against methicillin-resistant Staphylococcus aureus.

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2011-04-15 | E-BUGS-115 | biostudies-arrayexpress

Genomic variations define divergence of water/wildlife-associated Campylobacter jejuni niche specialists from common clonal complexes

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