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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human aortic endothelial cells, HAEC treated with TNF-alpha amplified aRNA samples vs unamplified RNA samples

ABSTRACT: Fidelity of TNF-alpha induced differential gene expression in amplified aRNA samples relative to unamplified RNA samples (human aortic endothelial cells, HAEC).

ORGANISM(S): Homo sapiens

SUBMITTER: Peter Davies

PROVIDER: E-CBIL-3 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Fidelity and enhanced sensitivity of differential transcription profiles following linear amplification of nanogram amounts of endothelial mRNA.

Polacek Denise C DC Passerini Anthony G AG Shi Congzhu C Francesco Nadeene M NM Manduchi Elisabetta E Grant Gregory R GR Powell Steven S Bischof Helen H Winkler Hans H Stoeckert Christian J CJ Davies Peter F PF

Physiological genomics 20030416 2

Although mRNA amplification is necessary for microarray analyses from limited amounts of cells and tissues, the accuracy of transcription profiles following amplification has not been well characterized. We tested the fidelity of differential gene expression following linear amplification by T7-mediated transcription in a well-established in vitro model of cytokine [tumor necrosis factor alpha (TNFalpha)]-stimulated human endothelial cells using filter arrays of 13,824 human cDNAs. Transcription ...[more]

PMID: 12700361

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Transcription profiling of pig aortic endothelium taken from regions of disturbed and undisturbed flow

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2006-07-21 | E-CBIL-17 | biostudies-arrayexpress

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Effects of model chylomicron remants on gene expresssion in human aortic endothelial cells (HAEC)

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2016-05-19 | E-GEOD-80067 | biostudies-arrayexpress

Transcription profiling of Ts65Dn vs control mice in nine different mouse tissues

Project description:To understand the molecular basis of Down syndrome pathogenesis, we performed a transcriptome analysis of nine different tissues in Ts65Dn, an established mouse model of human trisomy 21. Ts65Dn mice have segmental trisomy of mouse chromosome 16 with ca. 128 genes at dosage imbalance (Reeves et al. 1995). The Ts65Dn mouse is widely used as a model for studies of DS because it is at dosage imbalance for the orthologs of about half the 284 Chr21 genes. Ts65Dn mice have several features that directly parallel developmental anomalies of DS. We compare here the expression of 136 mouse orthologs of Chr21 genes, 77 of which are triplicated in Ts65Dn, in trisomic and euploid mice. <br> <br> We designed a mouse cDNA expression array interrogating 136 mmu21 genes. RNA pools from four adult male Ts65Dn mice and four male euploid littermates were prepared from cortex and dissected from three to four month-old mice. Directly labeled first strand cDNA probes from nine different tissues were hybridized to the arrays in replicated hybridizations. A total of 446 genes that are not triplicated in Ts65Dn mice served as controls. These included 62 mmu21 genes from MMU10, MMU17, and non-triplicated portions of MMU16, plus 384 randomly distributed mouse cDNAs from the Unigene collection.

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Comparison of genomic DNA amplification methods

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2024-04-09 | PXD043203 | Pride

Microbiota HAEC

Project description:Microbiota HAEC

| PRJEB55092 | ENA

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