Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse Ngn3 expressing cells and endocrine pancreatic cells from wild type and Ngn3 mutant animals at day 15 of embryonic development


ABSTRACT: The basic helix-loop-helix transcription factor Neurogenin3 (Ngn3/Neurog3) is expressed in endocrine progenitor cells in the embryonic mouse pancreas. Ngn3 controls endocrine cell fate decisions. Ngn3 deficient mice do not develop any pancreatic endocrine cells, including insulin producing beta cells, and die postnatally from diabetes. Therefore, the characterization of gene expression in Ngn3-expressing cells and their progeny is of particular interest for the development of novel strategies for cell replacement therapies in type-1 diabetes. Here we describe two studies. In the first study (8 assays) we used mice where the EYFP (Enhanced Yellow fluorescent Protein) is expressed under the control of Ngn3 regulatory elements (knock add on strategy). EYFP-positive, Ngn3-expressing cells, were FACS sorted from embryonic pancreas at day 15.5 (E15.5), as well as EYFP-negative cells. In the second study (6 assays) we compared wild-type and Ngn3 mutant pancreas at E15.5. All samples were hybridized to Affymetrix GeneChip Mouse Genome 430.2.0 array.

INSTRUMENT(S): Affymetrix Fluidics Station 400

ORGANISM(S): Mus musculus

SUBMITTER: Gerard Gradwohl 

PROVIDER: E-CBIL-48 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Rfx6 is an Ngn3-dependent winged helix transcription factor required for pancreatic islet cell development.

Soyer Josselin J   Flasse Lydie L   Raffelsberger Wolfgang W   Beucher Anthony A   Orvain Christophe C   Peers Bernard B   Ravassard Philippe P   Vermot Julien J   Voz Marianne L ML   Mellitzer Georg G   Gradwohl Gérard G  

Development (Cambridge, England) 20100101 2


The transcription factor neurogenin 3 (Neurog3 or Ngn3) controls islet cell fate specification in multipotent pancreatic progenitor cells in the mouse embryo. However, our knowledge of the genetic programs implemented by Ngn3, which control generic and islet subtype-specific properties, is still fragmentary. Gene expression profiling in isolated Ngn3-positive progenitor cells resulted in the identification of the uncharacterized winged helix transcription factor Rfx6. Rfx6 is initially expressed  ...[more]

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