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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse islet growth after partial pancreatectomy and exendin-4 Treatment

ABSTRACT: Diabetes mellitus results from an inadequately functioning beta-cell mass. In the adult pancreas, beta-cell mass is dynamic, increasing to meet metabolic demands and decreasing with metabolic or injury insults. Exendin-4 (Ex-4) is a glucagon-like peptide-1 receptor agonist that augments beta-cell mass by increasing beta-cell neogenesis and proliferation and by reducing apoptosis. We utilized a cDNA microarray approach to identify genes that are differentially regulated during islet growth after Ex-4 treatment or a partial pancreatectomy (Ppx). Mice underwent 50% Ppx or sham operation and received Ex-4 or vehicle every 24 hours. cDNA prepared from total pancreatic RNA isolated at 12, 24 and 48 hrs after surgery was hybridized to the PancChip 4.0 microarray.

ORGANISM(S): Mus musculus

SUBMITTER: Doris Stoffers

PROVIDER: E-CBIL-5 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of transcriptional targets during pancreatic growth after partial pancreatectomy and exendin-4 treatment.

De León Diva D DD Farzad Cyrus C Crutchlow Michael F MF Brestelli John J Tobias John J Kaestner Klaus H KH Stoffers Doris A DA

Physiological genomics 20060101 2

After partial pancreatectomy (Ppx), substantial regeneration of the endocrine and exocrine pancreatic compartments has been shown in adult rodents. Exendin-4 (Ex-4) is a glucagon-like peptide-1 receptor agonist that augments endocrine beta-cell mass by stimulating neogenesis, proliferation, and cell survival. After Ppx, treatment with Ex-4 ameliorates hyperglycemia by stimulating beta-cell mass recovery. We utilized a cDNA microarray approach to identify genes differentially regulated during pan ...[more]

PMID: 16410542

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