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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse NIH-3T3 cells treated with interferon alpha or gamma

ABSTRACT: Expression data from NIH-3T3 cells treated with mock, 100 U/ml IFN alpha or 100 U/ml gamma for 1 or 3h on nt-RNA labeled for 30-60 min at different times of interferon treatment; Differential gene expression caused by IFN alpha or gamma was analyzed in newly transcribed RNA (nt-RNA) of NIH-3T3 cells treated for 1 or 3 h. RNA was labeled for 30 to 60 min and separated from total cellular RNA (tc-RNA) following Trizol RNA preparation and thiol-specific biotinylation; We used microarrays to analyze the effects of IFNalpha and gamma treatment in newly transcribed RNA (nt-RNA) Experiment Overall Design: NIH-3T3 cells (5th to 15th passage after thawing) were split 24 h before start of the experiment. When starting the experiment 80% confluency was reached. The experiment was started by applying fresh, prewarmed, CO2-equilibrated medium containing either mock, 100U/ml IFN alpha or 100 U/ml IFN gamma. Labeling was either started simultaneously, 30 or 150 minutes later.

ORGANISM(S): Mus musculus

SUBMITTER: Lars DÃ¶lken

PROVIDER: E-GEOD-9975 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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newly transcribed RNA (nt-RNA) for IFN alpha and gamma time course

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