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Transcription profiling of mouse NIH-3T3 cells treated with mock, interferon alpha or gamma for 1or 3h

ABSTRACT: Differential gene expression caused by 1h and 3h of IFN alpha or gamma treatment was analyzed in total cellular RNA of NIH-3T3 cells compared to mock; We used microarrays to analyze the effects of 1 and 3h of IFNalpha and gamma treatment in total cellular RNA Experiment Overall Design: NIH-3T3 cells ( 5th to 15th passage after thawing) were split from confluent plates 24h before start of the experiment. At the begin of the experiment about 80% confluency was reached. The experiment was started by applying fresh, prewarmed, CO2-equilibrated medium containing either mock, 100U/ml IFN alpha or 100 U/ml IFN gamma. Labeling was either started together with addition of IFN for 60 minutes or for 30 minutes starting 150 minutes after begin of treatment.

ORGANISM(S): Mus musculus

SUBMITTER: Lars DÃ¶lken

PROVIDER: E-GEOD-9977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of mouse NIH-3T3 cells treated with interferon alpha or gamma

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