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Small RNAs in four Argonaute complexes in Arabidopsis

ABSTRACT: The core components of RNA silencing effector complexes include small RNAs and members of the Argonaute (AGO) protein family. Arabidopsis encodes ten AGOs and complex population of small RNAs. It remains largely unknown how these small RNAs are recognized and recruited by each AGO complex. Here we purified four AGO complexes and identified small RNAs in each complex by deep sequencing. Keywords: Small RNA sorting Small RNAs were prepared from Arabidopsis AGO1, AGO2, AGO4 and AGO5 complexes, ligated to a 3' adaptor and a 5' acceptor sequentially, and then RT-PCR amplified. PCR products were reamplified using a pair of solexa cloning primers and then provided for sequencing. For technical details, see Qi, Y., He, X., Wang, X.J., Kohany, O., Jurka, J., and Hannon, G.J. 2006. Distinct catalytic and non-catalytic roles of ARGONAUTE4 in RNA-directed DNA methylation. Nature 443(7114): 1008-1012.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Tao Cai 

PROVIDER: E-GEOD-10036 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide.

Mi Shijun S   Cai Tao T   Hu Yugang Y   Chen Yemiao Y   Hodges Emily E   Ni Fangrui F   Wu Liang L   Li Shan S   Zhou Huanyu H   Long Chengzu C   Chen She S   Hannon Gregory J GJ   Qi Yijun Y  

Cell 20080313 1

Argonaute (AGO) proteins recruit small RNAs to form the core of RNAi effector complexes. Arabidopsis encodes ten AGO proteins and a large network of small RNAs. How these small RNAs are sorted into specific AGO complexes remains largely unknown. We have cataloged small RNAs resident in four AGO complexes. We found that AGO2 and AGO4 preferentially recruit small RNAs with a 5' terminal adenosine, whereas AGO1 harbors microRNAs (miRNAs) that favor a 5' terminal uridine. AGO5 predominantly binds sm  ...[more]

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