Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human MCF7 cells following short-term E deprivation, ER-alpha siRNA knockdown and oxidant treatments


ABSTRACT: To investigate the oxidant sensitivity of E/ER regulated gene expression, E/ER regulated genes are identified using E deprivation or; ER-alpha siRNA knockdown; and oxidative stress responsive is determined by 8hr exposure to diamide, hydrogen peroxide and menadione Experiment Overall Design: MCF7 cells were treated under various conditions to identifiy oxidative stress responsive E/ER regulated genes. Association of these genes with respect to tumor phenotypes are assessed

ORGANISM(S): Homo sapiens

SUBMITTER: Christina Yau 

PROVIDER: E-GEOD-10061 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genes responsive to both oxidant stress and loss of estrogen receptor function identify a poor prognosis group of estrogen receptor positive primary breast cancers.

Yau Christina C   Benz Christopher C CC  

Breast cancer research : BCR 20080717 4


<h4>Introduction</h4>Oxidative stress can modify estrogen receptor (ER) structure and function, including induction of progesterone receptor (PR), altering the biology and clinical behavior of endocrine responsive (ER-positive) breast cancer.<h4>Methods</h4>To investigate the impact of oxidative stress on estrogen/ER-regulated gene expression, RNA was extracted from ER-positive/PR-positive MCF7 breast cancer cells after 72 hours of estrogen deprivation, small-interfering RNA knockdown of ER-alph  ...[more]

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