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Comparative expression analysis between TTHB212 deletion mutant and wild-type of T. thermophilus HB8

ABSTRACT: We observed the expression profile of the total mRNA of TTHB212 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70Â°C compared with that of wild type. Keywords: cell type comparison Three wild-type and mutant T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 5 h for wild-type and 7 h for TTHB212 deletion mutant, respectively, and then crude RNA was extracted. In order to compare mRNA expression in the TTHB212 deletion mutant with that of wild type, the expression of each mRNA was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

ORGANISM(S): Thermus thermophilus HB8

SUBMITTER: Akeo Shinkai

PROVIDER: E-GEOD-10371 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional identification of an anti-sigmaE factor from Thermus thermophilus HB8.

Sakamoto Keiko K Agari Yoshihiro Y Yokoyama Shigeyuki S Kuramitsu Seiki S Shinkai Akeo A

Gene 20080718 2

The TTHB212 gene from extremely thermophilic bacterium Thermus thermophilus HB8 forms an operon with the upstream sigE gene encoding an extracytoplasmic function sigma factor, sigma(E), the sole alternative sigma factor of this strain, on megaplasmid pTT27. The TTHB212 gene encodes a poorly conserved protein, which has been predicted to be a transmembrane one with N-terminal intracellular and C-terminal extracytoplasmic domains. The N-terminal domain of TTHB212 protein (TTHB212N) prevented sigma ...[more]

PMID: 18682280

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