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Time course of bacteriophage ?YS40 infection in wild-type and crp deletion Thermus thermophilus HB8 strain

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE16954: Time course of the mRNA expression after bacteriophage ?YS40 infection in wild-type Thermus thermophilus HB8 strain. GSE16955: Time course of the mRNA expression after phage ?YS40 infection in crp deletion mutant of Thermus thermophilus HB8. Refer to individual Series

ORGANISM(S): Thermus thermophilus HB8

SUBMITTER: Yoshihiro Agari

PROVIDER: E-GEOD-16978 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profile of Thermus thermophilus CRISPR systems after phage infection.

Agari Yoshihiro Y Sakamoto Keiko K Tamakoshi Masatada M Oshima Tairo T Kuramitsu Seiki S Shinkai Akeo A

Journal of molecular biology 20091103 2

The clustered regularly interspaced short palindromic repeat (CRISPR) systems composed of DNA direct repeats designated as CRISPRs and several CRISPR-associated (cas) genes, which are present in many prokaryotic genomes, make up a host defense system against invading foreign replicons such as phages. In order to investigate the altered expression profiles of the systems after phage infection using a model organism, Thermus thermophilus HB8, which has 12 CRISPR loci, genome-wide transcription pro ...[more]

PMID: 19891975

Similar Datasets

Time course of the mRNA expression after phage ϕYS40 infection in crp deletion mutant of Thermus thermophilus HB8.

Project description:We observed the expression profile of the total mRNA in crp (TTHA1437) deletion mutant strain of Thermus thermophilus HB8 during infection of bacteriophage ϕYS40. Keywords: time course, bacteriophage, infection, CRP, cAMP receptor protein, deletion mutant Three crp (TTHA1437) delection mutant strain of T. thermophilus HB8 were pre-cultured at 70 C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70°C until A600 value being ~0.8 (1.7 108 cells/ml) that corresponds to logarithmic growth phase. Then the FYS40 phage was infected to the medium at multiplicity of infection (m.o.i.) being ~1, and continued cultivation. Cells were collected after 0, 75 and 100 min infection, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample.

2009-11-02 | E-GEOD-16955 | biostudies-arrayexpress

Time course of the mRNA expression after bacteriophage ÏYS40 infection in wild-type Thermus thermophilus HB8 strain.

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain during infection of bacteriophage ÏYS40. Keywords: time course, bacteriophage, infection, wild type Three wild-type T. thermophilus HB8 strains were pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C until A600 value being ~0.8 (1.7 Ã 108 cells/ml) that corresponds to logarithmic growth phase. Then the Î¦YS40 phage was infected to the medium at multiplicity of infection (m.o.i.) being ~1, and continued cultivation. Cells were collected after 0, 25, 50, 75 and 100 min infection, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample.

2010-06-20 | E-GEOD-16954 | biostudies-arrayexpress

Time course of the mRNA expression after phage ÏYS40 infection in crp deletion mutant of Thermus thermophilus HB8

Project description:We observed the expression profile of the total mRNA in crp (TTHA1437) deletion mutant strain of Thermus thermophilus HB8 during infection of bacteriophage ÏYS40. Three crp (TTHA1437) delection mutant strain of T. thermophilus HB8 were pre-cultured at 70 C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C until A600 value being ~0.8 (1.7 108 cells/ml) that corresponds to logarithmic growth phase. Then the FYS40 phage was infected to the medium at multiplicity of infection (m.o.i.) being ~1, and continued cultivation. Cells were collected after 0, 75 and 100 min infection, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip..

2010-10-15 | E-GEOD-21474 | biostudies-arrayexpress

SuperSeries for the study of expression analysis of the SdrP (TTHA1359) in T. thermophilus HB8

Project description:This SuperSeries is composed of the following subset Series: GSE10368: Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium. GSE10369: comparative expression analysis between TTHA1359 deletion mutant and wild-type of T. thermophilus HB8 GSE11671: comparative expression analysis between logarithmic and stationary phase of T. thermophilus HB8 on a synthetic medium. Keywords: SuperSeries Refer to individual Series

2010-05-25 | E-GEOD-10370 | biostudies-arrayexpress

SuperSeries for the study of expression analysis of the T. thermophilus CRP (TTHA1437)

Project description:This SuperSeries is composed of the following subset Series: GSE7165: mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium. GSE7166: mRNA expression in crp (TTHA1437) deletion mutant of Thermus thermophilus HB8 strain grown on a rich medium Keywords: SuperSeries Refer to individual Series

2010-05-27 | E-GEOD-7175 | biostudies-arrayexpress

Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium.

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70Â°C. Keywords: time course Three wild-type T. thermophilus HB8 strains were pre-cultured at 70Â°C for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2010-05-25 | E-GEOD-10368 | biostudies-arrayexpress

SuperSeries for the study of expression analysis of the T. thermophilus sigE (TTHB211)

Project description:This SuperSeries is composed of the following subset Series: GSE7165: mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium. GSE8780: mRNA expression in sigE (TTHB211) deletion mutant of Thermus thermophilus HB8 strain grown on a rich medium Keywords: SuperSeries Refer to individual Series

2007-09-04 | E-GEOD-8781 | biostudies-arrayexpress

Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium (labeling:ENZO)

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC. Keywords: time course Three wild-type T. thermophilus HB8 strains were pre-cultured at 70oC for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70oC. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2010-05-27 | E-GEOD-7165 | biostudies-arrayexpress

Comparative expression analysis between CRP (TTHA1437) deletion mutant and wild-type of T. thermophilus HB8

Project description:We observed the expression profile of the total mRNA of crp (TTHA1437) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC compared with that of wild type. Keywords: cell type comparison Three wild-type and mutant T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70oC. Cells were collected after 6 h and 8 h, and then crude RNA was extracted. In order to compare mRNA expression in the crp (TTHA1437) deletion mutant with that of wild type, the expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2010-05-27 | E-GEOD-7166 | biostudies-arrayexpress

Comparative expression analysis between TTHB212 deletion mutant and wild-type of T. thermophilus HB8

Project description:We observed the expression profile of the total mRNA of TTHB212 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70Â°C compared with that of wild type. Keywords: cell type comparison Three wild-type and mutant T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 5 h for wild-type and 7 h for TTHB212 deletion mutant, respectively, and then crude RNA was extracted. In order to compare mRNA expression in the TTHB212 deletion mutant with that of wild type, the expression of each mRNA was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2010-05-25 | E-GEOD-10371 | biostudies-arrayexpress

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