Time course of the mRNA expression after bacteriophage ϕYS40 infection in wild-type Thermus thermophilus HB8 strain.

ABSTRACT: We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain during infection of bacteriophage ϕYS40. Keywords: time course, bacteriophage, infection, wild type Three wild-type T. thermophilus HB8 strains were pre-cultured at 70°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70°C until A600 value being ~0.8 (1.7 × 108 cells/ml) that corresponds to logarithmic growth phase. Then the ΦYS40 phage was infected to the medium at multiplicity of infection (m.o.i.) being ~1, and continued cultivation. Cells were collected after 0, 25, 50, 75 and 100 min infection, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample.

ORGANISM(S): Thermus thermophilus HB8

SUBMITTER: Akeo Shinkai 

PROVIDER: E-GEOD-16954 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress