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Transcription profiling of mouse MALP-2-stimulated macrophages FROM wild-TYPE, IRAK-2-/- AND IRAK-1-/IRAK-2-/- animals

ABSTRACT: IL-1R-associated kinases (IRAKs) participate in Toll-like receptor (TLR) signal transduction. MALP-2 is a TLR2 ligand, and stimulation of macrophages with MALP-2 activates expression of various genes including proinflammatory cytokines. We used microarrays to examine influence of IRAK-2 or IRAK-1/IRAK-2 deficiency in MALP-2-inducible gene expression. Experiment Overall Design: Peritoneal macrophages from wild-type, IRAK-2-/- and IRAK-1/IRAK-2 mice were stimulated with MALP-2 for 0, 2, 4, and 8 hours, followed by RNA extraction. Then hybridization on affymetrix microarrays was performed.

ORGANISM(S): Mus musculus

SUBMITTER: Osamu Takeuchi

PROVIDER: E-GEOD-10765 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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