Unknown,Transcriptomics,Genomics,Proteomics

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Real-time quantitative PCR analysis of ARF-deficient macrophages

ABSTRACT: Macrophages are a major cellular component of all inflammatory situations, generating proinflammatory cytokines such as TNF-alpha, IL-1, and IL-6 that are central to the initiation and maintenance of inflammation. To determine whether the tumor suppressor ARF plays a role in inflammatory gene expression, we used an 84-gene RT2 PCR array to examine the expression of inflammation-associated genes in WT and ARF-deficient macrophages treated with the TLR4 ligand LPS. Peritoneal macrophages from WT and ARF-deficient mice were obtained and treated with LPS (200ng/ml) for 4 hours. WT control (without stimulation n=4), WT LPS (n=4), ARF Control (n=4), ARF LPS (n=4)

ORGANISM(S): Mus musculus

SUBMITTER: Sonsoles Hortelano

PROVIDER: E-GEOD-32446 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The tumor suppressor ARF regulates innate immune responses in mice.

Través Paqui G PG López-Fontal Raquel R Luque Alfonso A Hortelano Sonsoles S

Journal of immunology (Baltimore, Md. : 1950) 20111116 12

The innate immune system is the first line of defense against invading organisms, and TLRs are the main sensors of microbial components, initiating signaling pathways that induce the production of proinflammatory cytokines and type I IFNs. An antiviral action for the tumor suppressor alternative reading frame (ARF) has been reported; however, the precise role of ARF in innate immunity is unknown. In this study, we show that ARF plays an important role in regulation of inflammatory responses. In ...[more]

PMID: 22095712

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Dataset Information

Real-time quantitative PCR analysis of ARF-deficient macrophages

Publications

The tumor suppressor ARF regulates innate immune responses in mice.

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