Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Drosophila adult females Logjam p24 mutant to test the hypothesis that loss of loj function causes a transcriptional response characteristic of ER stress activation

ABSTRACT: We performed genome-wide profiling experiments on tissues from Drosophila melanogaster adult females with a mutation in the p24 gene logjam (loj) to test the hypothesis that loss of loj function causes a transcriptional response characteristic of ER stress activation. Experiment Overall Design: We compared expression profiles from loj mutant females to those of controls. We compared mutant and control abdomen tissue as well as mutant and control tissue preparations from the remainder of the fly (the head/thorax). Three mutant and three control RNA samples for each set of tissue were used for array hybridization (12 total array samples).

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Ginger Carney

PROVIDER: E-GEOD-10940 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Loss of p24 function in Drosophila melanogaster causes a stress response and increased levels of NF-kappaB-regulated gene products.

Boltz Kara A KA Carney Ginger E GE

BMC genomics 20080508

<h4>Background</h4>Secretory and transmembrane proteins traverse the endoplasmic reticulum (ER) and Golgi compartments for final maturation prior to reaching their functional destinations. Members of the p24 protein family, which are transmembrane constituents of ER and Golgi-derived transport vesicles, function in trafficking some secretory proteins in yeast and higher eukaryotes. Yeast p24 mutants have minor secretory defects and induce an ER stress response that likely results from accumulati ...[more]

PMID: 18466616

Similar Datasets

Transcription profiling by array of Drosophila head and thorax from females mated with sex peptide null or sex peptide producing males

Project description:Comparison of females mated to males null for Sex-Peptide (SP0, Liu, H. and E. Kubli, Sex-peptide is the molecular basis of the sperm effect in Drosophila melanogaster. Proc Natl Acad Sci U S A, 2003. 100(17): p. 9929-33) or to control, Sex-Peptide producing, males. Comparisons were made at 3 and 6 hours after mating, in dissected Head-Thorax body parts.

2009-08-01 | E-MEXP-2070 | biostudies-arrayexpress

Transcription profiling of Drosophila abdomen from females mated with sex peptide null or sex peptide producing males

2009-08-01 | E-MEXP-2074 | biostudies-arrayexpress

MicroRNA profiling of Drosophila head and thorax from females mated with sex peptide null or sex peptide producing males

2009-08-01 | E-MEXP-2079 | biostudies-arrayexpress

MicroRNA profiling of Drosophila abdomen from females mated with sex peptide null or sex peptide producing males

2009-08-01 | E-MEXP-2080 | biostudies-arrayexpress

Transcription profiling of Drosophila melanogaster females head+thorax vs abdomen organism parts and high mating pressure vs low mating pressure to investigate the genomic transcriptional signature of mating costs

Project description:In this study we examined the genomic transcriptional signature of mating costs in female fruitflies in which mating rate was elevated 3-4 fold. We conducted 4-fold biological replication and randomly sampled cohorts of females before the major mortality effects of the elevated mating appeared (to avoid the potentially biasing effects of lineage selection). We then assayed differential gene expression, in two different body parts (abdomen versus head+thorax), using genome wide microarrays.

2010-09-30 | E-TABM-800 | biostudies-arrayexpress

Proteomic comparison of summer and winter Apis mellifera workers shows reduced protein abundance in winter samples

Project description:Apis mellifera workers in temperate climates display two castes; short lived summer bees that engage in nursing, hive maintenance and foraging, and long lived winter bees (diutinus bees) which remain within the hive and are essential for thermoregulation. Label free quantitative proteomic analysis was conducted on A. mellifera workers sampled in June and December to compare the proteomes of summer and winter bees. Proteomic analysis was completed on head, abdominal and venom sac samples which revealed an elevated level of protein abundance in summer bees but and a decrease in protein abundance in winter bees. Head and abdominal samples displayed an increase in cuticular proteins in summer samples whereas an increase in xenobiotic proteins was observed in winter samples. Several carbohydrate metabolism pathways which have been linked to energy production and longevity in insects were observed to be increased in abundance in winter samples in comparison to summer samples. Proteomic analysis of the venom sacs an increased abundance and expression of bee venom associated proteins in comparison to winter workers. These data provides an insight into the adaptions of A. mellifera workers in summer and winter and may aid in future treatment and disease studies on honeybee colonies.

2022-05-20 | PXD030483 | Pride

Proteomic comparison of summer and winter Apis mellifera workers shows reduced protein abundance in winter samples

2022-11-21 | PXD030481 | Pride

Spatially differential regulation of ATF2 phosphorylation contributes to warning coloration of gregarious locusts

Project description:Aposematism and warning coloration are common defense strategies used by animals to deter predators. Pestilential gregarious locusts display a striking black-brown pattern, which is presumed to be a form of warning coloration compared to green solitary locusts. However, the specific mechanisms involved in regulating this distinctive pattern are not yet understood. Here, we found that different amounts of β-carotene and β-carotene-binding protein (βCBP) complexes confer the black tergum and brown sternum of locust. The expression level of βCBP is regulated by the bZIP transcription factor ATF2, which is activated by protein kinase C alpha (PKCα) in response to crowding. Specifically, ATF2 is phosphorylated at Ser327 and translocates to the nucleus, where it binds to specific sites on the βCBP promoter and stimulates overexpression. Differential phosphorylation of ATF2 resulted in the divergent black and brown body coloration of gregarious locusts. The overexpression of βCBP in the sternum is essential for the accumulation of red pigments, which creates the sharp contrast between the black tergum and the brown sternum. This spatial variation in ATF2 phosphorylation levels allows locusts to adapt to changing environmental conditions and better evade predation.

2023-08-24 | PXD042860 | Pride

The life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts

Project description:Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used whole-cell quantitative proteomics to track the protein composition of sperm across three female reproductive tissues (bursa, seminal receptacle, and spermatheca) and three post-mating timepoints (30 minutes, 2 hours, and 4 days). In combination with an update and expansion of the seminal vesicle sperm proteome and sex-specific isotopic labeling to identify female-contributed sperm proteins, our data provide a comprehensive, quantitative analysis of the molecular life history of Drosophila sperm.

2022-03-14 | PXD027807 | Pride

RNA-seq of wild type and mutant Drosophila flies with a deletion of the gene CG16717 (dMPPED)

Project description:Flies deficient in the gene CG16716 were created and showed a reduced life span when compared to wild type w1118 flies. Flies were harvested on day 35 (both wild type and mutant flies), RNA prepared and subjected to RNA-seq.

2021-03-31 | E-MTAB-9081 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data