Proteomics

Dataset Information

0

Proteomic comparison of summer and winter Apis mellifera workers shows reduced protein abundance in winter samples


ABSTRACT: Apis mellifera workers in temperate climates display two castes; short lived summer bees that engage in nursing, hive maintenance and foraging, and long lived winter bees (diutinus bees) which remain within the hive and are essential for thermoregulation. Label free quantitative proteomic analysis was conducted on A. mellifera workers sampled in June and December to compare the proteomes of summer and winter bees. Proteomic analysis was completed on head, abdominal and venom sac samples which revealed an elevated level of protein abundance in summer bees but and a decrease in protein abundance in winter bees. Head and abdominal samples displayed an increase in cuticular proteins in summer samples whereas an increase in xenobiotic proteins was observed in winter samples. Several carbohydrate metabolism pathways which have been linked to energy production and longevity in insects were observed to be increased in abundance in winter samples in comparison to summer samples. Proteomic analysis of the venom sacs an increased abundance and expression of bee venom associated proteins in comparison to winter workers. These data provides an insight into the adaptions of A. mellifera workers in summer and winter and may aid in future treatment and disease studies on honeybee colonies.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Apis Mellifera (honeybee)

SUBMITTER: Rachel Ward  

LAB HEAD: Kevin Kavanagh

PROVIDER: PXD030483 | Pride | 2022-05-20

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
Abdomen_MaxQuant_Output.zip Other
Abdomen_experimentalDesignTemplate.txt Txt
Head_MaxQuant_Output.zip Other
Head_experimentalDesignTemplate.txt Txt
Summer_Abd_1.raw Raw
Items per page:
1 - 5 of 24
altmetric image

Publications

Proteomic analysis of summer and winter Apis mellifera workers shows reduced protein abundance in winter samples.

Ward Rachel R   Coffey Mary M   Kavanagh Kevin K  

Journal of insect physiology 20220507


Apis mellifera workers display two stages; short lived summer bees that engage in nursing, hive maintenance and foraging, and long lived winter bees (diutinus bees) which remain within the hive and are essential for thermoregulation and rearing the next generation of bees in spring before dying. Label free quantitative proteomic analysis was conducted on A. mellifera workers sampled in June and December to compare the proteomes of summer and winter bees. Proteomic analysis was performed on head,  ...[more]

Similar Datasets

2022-11-21 | PXD030481 | Pride
2022-11-21 | PXD032755 | Pride
2023-06-23 | PXD030369 | Pride
2012-05-23 | GSE25455 | GEO
2008-07-29 | E-MEXP-1044 | biostudies-arrayexpress
2024-04-23 | PXD047955 | Pride
2011-08-31 | E-GEOD-25455 | biostudies-arrayexpress
2008-12-09 | E-MEXP-1549 | biostudies-arrayexpress
2008-07-11 | E-MEXP-1552 | biostudies-arrayexpress
2008-12-31 | E-MEXP-1724 | biostudies-arrayexpress