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Transcriptome analysis of wild type and erythromycin-producing strain of S. erythraea

ABSTRACT: Erythromycin is a medically important antibiotic, biosynthesized by the actinomycete Saccharopolyspora erythraea. We used transcriptomic approach to compare whole genome expression in erythromycin high-producing strain, compared to the wild type S. erythraea strain in four stages of fermentation.

ORGANISM(S): Saccharopolyspora erythraea

PROVIDER: GSE65007 | GEO | 2016/06/22

SECONDARY ACCESSION(S): PRJNA272711

REPOSITORIES: GEO

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Transcriptome analysis of wild type and erythromycin-producing strain of S. erythraea

Project description:Erythromycin is a medically important antibiotic, biosynthesized by the actinomycete Saccharopolyspora erythraea. We used transcriptomic approach to compare whole genome expression in erythromycin high-producing strain, compared to the wild type S. erythraea strain in four stages of fermentation. 2 strains (3 individual fermentations each), 4 time points --> 24 samples (2 exluded from anaysis, 22 remaining); one color design

2016-06-22 | E-GEOD-65007 | biostudies-arrayexpress

Phenotype and expression profile of S. erythraea rifampicin-resistant (rif) mutants affected in erythromycin production

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Project description:Abstract: Transcript levels in production cultures of wildtype and classically improved strains of the actinomycete bacteria Saccharopolyspora erythraea and Streptomyces fradiae were monitored using microarrays of the sequenced actinomycete S. coelicolor. Sac. erythraea and S. fradiae synthesize the polyketide antibiotics erythromycin and tylosin, respectively, and the classically improved strains contain unknown overproduction mutations. The Sac. erythraea overproducer was found to express the entire 56-kb erythromycin gene cluster several days longer than the wildtype strain. In contrast, the S. fradiae wildtype and overproducer strains expressed the 85-kb tylosin biosynthetic gene cluster similarly, while they expressed several tens of other S. fradiae genes and S. coelicolor homologs differently, including the acyl-CoA dehydrogenase gene aco and the S. coelicolor isobutyryl-CoA mutase homolog icmA. These observations indicated that overproduction mechanisms in classically improved strains can affect both the timing and rate of antibiotic synthesis, and alter the regulation of antibiotic biosynthetic enzymes and enzymes involved in precursor metabolism. This SuperSeries is composed of the SubSeries listed below.

2006-03-08 | GSE4395 | GEO

Phenotype and expression profile of S. erythraea rifampicin-resistant (rif) mutants affected in erythromycin production

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Saccharopolyspora erythraea RNA-seq

Project description:We report the high-throughput profiling of saccharopolyspora erythraea including a industrial strain HL3168 E3 and a wild-type strain NRRL23338. The aim was to evaluate the difference in expression of sRNA predicted in silico related to secondary metabolites in Saccharopolyspora erythraea.

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Saccharopolyspora erythraea RNA-seq

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Control of chitin and N-acetylglucosamine utilization in Saccharopolyspora erythraea

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