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Global gene expression analysis of Arabidopsis ppi2-1 mutant


ABSTRACT: This study aims at investigating the gene expression profile of Arabidopsis ppi2-1 mutant. Using Super-SAGE, we compared the gene expression profile of ppi2-1 mutant with that of wild-type Arabidopsis. To obtain the SuperSAGE libraries, we followed the original SuperSAGE protocol described by Matsumura and colleagues (Matsumura et al., 2003) with some modification. Total RNA was isolated from the 3rd to 6th leaves of Ws and the ppi2-1 mutant using RNAiso reagent. The SuperSAGE library was directly sequenced with a large-scale pyrosequencing method.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Takehito Inaba 

PROVIDER: E-GEOD-12325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Coordination of plastid protein import and nuclear gene expression by plastid-to-nucleus retrograde signaling.

Kakizaki Tomohiro T   Matsumura Hideo H   Nakayama Katsuhiro K   Che Fang-Sik FS   Terauchi Ryohei R   Inaba Takehito T  

Plant physiology 20090902 3


Expression of nuclear-encoded plastid proteins and import of those proteins into plastids are indispensable for plastid biogenesis. One possible cellular mechanism that coordinates these two essential processes is retrograde signaling from plastids to the nucleus. However, the molecular details of how this signaling occurs remain elusive. Using the plastid protein import2 mutant of Arabidopsis (Arabidopsis thaliana), which lacks the atToc159 protein import receptor, we demonstrate that the expre  ...[more]

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