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Transcription profiling of Arabidopsis seed compartments at the pre-globular stage

ABSTRACT: We collected pre-globular stage seed compartments from 7-micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments of seeds containing pre-globular-stage embryos was identified as those seeds containing embryo propers made up of between 2 and 8 cells. For the purposes of this study we captured 6 compartments: embyro proper, micropylar endosperm, peripheral endosperm, chalazal endosperm, chalazal seed coat and general seed coat. Serial sections of entire seeds were also captured for comparison. Experiment Overall Design: Pre-globular stage seed compartments or whole seeds were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripheral endosperm, chalazal endosperm, general seed coat, chalazal seed coat, whole seeds, 2 biological replicates each).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ryan Christopher Kirkbride 

PROVIDER: E-GEOD-12402 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The seed composition of Arabidopsis mutants for the group 3 sulfate transporters indicates a role in sulfate translocation within developing seeds.

Zuber Hélène H   Davidian Jean-Claude JC   Aubert Grégoire G   Aimé Delphine D   Belghazi Maya M   Lugan Raphaël R   Heintz Dimitri D   Wirtz Markus M   Hell Rüdiger R   Thompson Richard R   Gallardo Karine K  

Plant physiology 20100811 2

Sulfate is required for the synthesis of sulfur-containing amino acids and numerous other compounds essential for the plant life cycle. The delivery of sulfate to seeds and its translocation between seed tissues is likely to require specific transporters. In Arabidopsis (Arabidopsis thaliana), the group 3 plasmalemma-predicted sulfate transporters (SULTR3) comprise five genes, all expressed in developing seeds, especially in the tissues surrounding the embryo. Here, we show that sulfur supply to  ...[more]

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