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Transcription profiling of Arabidopsis seed compartments at the linear-cotyledon stage

ABSTRACT: We collected linear-cotyledon stage seed compartments from 5 to 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing linear-coyledon-stage embryos. For the purposes of this study we captured 5 compartments: embyro proper, cellularized endosperm, chalazal endosperm, chalazal seed coat and general seed coat. Experiment Overall Design: linear-cotyledon stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 10 samples (embryo proper, micropylar endosperm, cellularized endosperm, general seed coat and chalazal seed coat, 2 biological replicates each ).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ryan Christopher Kirkbride

PROVIDER: E-GEOD-12403 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The seed composition of Arabidopsis mutants for the group 3 sulfate transporters indicates a role in sulfate translocation within developing seeds.

Zuber Hélène H Davidian Jean-Claude JC Aubert Grégoire G Aimé Delphine D Belghazi Maya M Lugan Raphaël R Heintz Dimitri D Wirtz Markus M Hell Rüdiger R Thompson Richard R Gallardo Karine K

Plant physiology 20100811 2

Sulfate is required for the synthesis of sulfur-containing amino acids and numerous other compounds essential for the plant life cycle. The delivery of sulfate to seeds and its translocation between seed tissues is likely to require specific transporters. In Arabidopsis (Arabidopsis thaliana), the group 3 plasmalemma-predicted sulfate transporters (SULTR3) comprise five genes, all expressed in developing seeds, especially in the tissues surrounding the embryo. Here, we show that sulfur supply to ...[more]

PMID: 20702726

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