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Global gene expression analysis of undifferentiated H1 hESC, and hEB cells differentiated with SF BVF2H conditions

ABSTRACT: Global gene expression analysis of human embryoid body (hEB) differentiations during differentiation in serum free conditions in the presence of BMP4, VEGF, and FGF2 growth factors A total of 5 samples are analyzed. Each sample is taken at each time point. There is no replicate. H1_hESC_day0 sample is used as control to compare to other samples Keywords: Cell differentiation Cell differentiation compares to undifferentiated status in a time-course of 3-, 5-, 7- and 10-day without replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Wayne Yu

PROVIDER: E-GEOD-12531 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression of angiotensin-converting enzyme (CD143) identifies and regulates primitive hemangioblasts derived from human pluripotent stem cells.

Zambidis Elias T ET Park Tea Soon TS Yu Wayne W Tam Ada A Levine Michal M Yuan Xuan X Pryzhkova Marina M Péault Bruno B

Blood 20080826 9

We report that angiotensin-converting enzyme (ACE), a critical physiologic regulator of blood pressure, angiogenesis, and inflammation, is a novel marker for identifying hemangioblasts differentiating from human embryonic stem cells (hESC). We demonstrate that ACE+CD45-CD34+/- hemangioblasts are common yolk sac (YS)-like progenitors for not only endothelium but also both primitive and definitive human lymphohematopoietic cells. Thrombopoietin and basic fibroblast growth factor are identified as ...[more]

PMID: 18728246

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