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Expression data from trisomy 21 and euploid induced pluripotent stem cell hematopoietic progenitors

ABSTRACT: We modeled human Trisomy 21 primitive hematopoiesis using induced pluripotent stem cells (iPSCs). Primitive multipotent progenitor populations generated from Trisomy 21 iPSCs showed normal proliferative capacity and megakaryocyte production, enhanced erythropoiesis and reduced myeloid development compared to euploid iPSCs. CD41+/235+ iPSC-derived progenitor cells were flow cytometrically sorted for RNA extraction and hybridization onto Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Ross Hardison

PROVIDER: E-GEOD-35561 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Trisomy 21-associated defects in human primitive hematopoiesis revealed through induced pluripotent stem cells.

Chou Stella T ST Byrska-Bishop Marta M Tober Joanna M JM Yao Yu Y Vandorn Daniel D Opalinska Joanna B JB Mills Jason A JA Choi John Kim JK Speck Nancy A NA Gadue Paul P Hardison Ross C RC Nemiroff Richard L RL French Deborah L DL Weiss Mitchell J MJ

Proceedings of the National Academy of Sciences of the United States of America 20121008 43

Patients with Down syndrome (trisomy 21, T21) have hematologic abnormalities throughout life. Newborns frequently exhibit abnormal blood counts and a clonal preleukemia. Human T21 fetal livers contain expanded erythro-megakaryocytic precursors with enhanced proliferative capacity. The impact of T21 on the earliest stages of embryonic hematopoiesis is unknown and nearly impossible to examine in human subjects. We modeled T21 yolk sac hematopoiesis using human induced pluripotent stem cells (iPSCs ...[more]

PMID: 23045704

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