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Transcription profiling by array of human MCF10A cells reveal changes upon expression of activated versions of MEK1 and HRAS

ABSTRACT: MCF10A cells were then transfected with MEK1(S217S221), HRAS(G12V), and null control vectors; Cells were lysed 24 hours post-transfection with collection of total RNA and protein Experiment Overall Design: Overexpression of Oncogenic protein was confirmed via Western blot

ORGANISM(S): Homo sapiens

SUBMITTER: Tom Januario

PROVIDER: E-GEOD-12764 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TRPS1 targeting by miR-221/222 promotes the epithelial-to-mesenchymal transition in breast cancer.

Stinson Susanna S Lackner Mark R MR Adai Alex T AT Yu Nancy N Kim Hyo-Jin HJ O'Brien Carol C Spoerke Jill J Jhunjhunwala Suchit S Boyd Zachary Z Januario Thomas T Newman Robert J RJ Yue Peng P Bourgon Richard R Modrusan Zora Z Stern Howard M HM Warming Søren S de Sauvage Frederic J FJ Amler Lukas L Yeh Ru-Fang RF Dornan David D

Science signaling 20110614 177

The basal-like subtype of breast cancer has an aggressive clinical behavior compared to that of the luminal subtype. We identified the microRNAs (miRNAs) miR-221 and miR-222 (miR-221/222) as basal-like subtype-specific miRNAs and showed that expression of miR-221/222 decreased expression of epithelial-specific genes and increased expression of mesenchymal-specific genes, and increased cell migration and invasion in a manner characteristic of the epithelial-to-mesenchymal transition (EMT). The tr ...[more]

PMID: 21673316

Publication: 1/2

Similar Datasets

Gene expression changes upon expression of activated versions of MEK1 and HRAS in MCF10A cells

Project description:MCF10A cells were then transfected with MEK1(S217S221), HRAS(G12V), and null control vectors Cells were lysed 24 hours post-transfection with collection of total RNA and protein Keywords: Oncogene inducation of gene expression changes

2009-01-12 | GSE12764 | GEO

Transcription profiling of human CD34+ derived neutrophil progenitors from umbilical cord blood, in response to Mek1 activation

Project description:analysis of differential gene expression in CD34+ derived neutrophil progenitors from umbilical cord blood, in response to Mek1 activation

2009-08-14 | E-TABM-754 | biostudies-arrayexpress

Histone H3 threonine 11 phosphorylation is catalyzed directly by the meiosis-specific kinase Mek1 and provides a molecular readout of Mek1 activity in vivo

Project description:Saccharomyces cerevisiae Mek1 is a CHK2/Rad53-family kinase that regulates meiotic recombination and progression upon its activation in response to DNA double-strand breaks (DSBs). The full catalog of direct Mek1 phosphorylation targets remains unknown. Here, we show that phosphorylation of histone H3 on threonine 11 (H3 T11ph) is induced by meiotic DSBs in S. cerevisiae and Schizosaccharomyces pombe. Molecular genetic experiments in S. cerevisiae confirmed that Mek1 is required for H3 T11ph and revealed that phosphorylation is rapidly reversed when Mek1 kinase is no longer active. Reconstituting histone phosphorylation in vitro with recombinant proteins demonstrated that Mek1 directly catalyzes H3 T11 phosphorylation. Mutating H3 T11 to nonphosphorylatable residues conferred no detectable meiotic defects, indicating that H3 T11ph is dispensable for Mek1 functions in controlling recombination. However, H3 T11ph provides an excellent marker of ongoing Mek1 kinase activity in vivo. Anti-H3 T11ph chromatin immunoprecipitation followed by deep sequencing demonstrated that H3 T11ph was highly enriched at presumed sites of attachment of chromatin to chromosome axes, gave a more modest signal along chromatin loops, and was present at still lower levels immediately adjacent to DSB hotspots. These localization patterns closely tracked the distribution of Red1 and Hop1, axis proteins required for Mek1 activation. These findings provide insight into the spatial disposition of Mek1 kinase activity and the higher order organization of recombining meiotic chromosomes.

2017-10-17 | GSE103823 | GEO

Transcription profiling by array of the influence of RAS mutants on the TGFB1-induced phenotype of PANC-1 cells

Project description:In brief, PANC-1 cells stably transfected with a dominant negative HRAS(S17N) mutant, a constitutively active KRAS2(G12V) mutant, or mock transfected with an EGFP expression construct were treated with TGFB1 or left untreated, respectively. Three independent experiments each were analyzed in duplicate.

2015-02-27 | E-MTAB-3364 | biostudies-arrayexpress

Gene expression data from IMR90 Control, IMR90 HRAS-G12V, IMR90 HRAS-G12V+shDOT1L, IMR90 DOT1L Overexpression

Project description:Gene expression data from IMR90 Control, IMR90 HRAS-G12V, IMR90 HRAS-G12V+shDOT1L, IMR90 DOT1L Overexpression

| PRJNA658579 | ENA

Spo11-oligo mapping in S. cerevisiae red1, hop1, mek1 mutants

Project description:Meiotic recombination starts with the formation of DNA double-strand breaks (DSBs) made by Spo11. In Saccharomyces cerevisiae, the nonrandom distribution of meiotic DSBs along the genome can be attributed to the combined influence of multiple factors on Spo11 cleavage. One factor is higher-order chromatin structure, particularly the loop-axis organization of meiotic chromosomes. Axial element proteins Red1 and Hop1 provide the basis for meiotic loop-axis organization and are implicated in diverse aspects of meiotic recombination. Mek1 is a meiotic-specific kinase associated with Red1 and Hop1. Red1, Hop1, and Mek1 are required for normal DSB levels, but their effects on the DSB distribution has not been examined, and exactly how these proteins influence DSB levels and distribution is unknown. Here, we examined the contributions of Red1, Hop1, and Mek1 to the DSB distribution by deep sequencing and mapping Spo11-associated oligonucleotides from red1, hop1, and mek1 mutant strains, thereby generating genome-wide meiotic DSB maps.

2018-09-01 | GSE84859 | GEO

Transcription profiling by array of human MCF10A cells over-expressing HRas or MEK1

Project description:MCF10A cells were then transfected with MEK1(S217S221), HRAS(G12V), and null control vectors; Cells were lysed 24 hours post-transfection with collection of total RNA and protein Experiment Overall Design: Overexpression of Oncogenic protein was confirmed via Western blot

| S-ECPF-GEOD-12764 | biostudies-other

Functional robustness of spermatogonial stem cells after induction of hyperactive Hras

Project description:We report on gene expression in stem cell-enriched, undifferentiated spermatogonia isolated from the adult mouse testis, in which one group are wild type and the other group contain a heterozygous Hras G12V mutation.

2019-04-18 | GSE129493 | GEO

MEK1/2 inhibitors unlock the constrained interferon response in macrophages through IRF1 signaling

Project description:Analysis of bone-marrow derived macrophages (BMDMs) stimulated with single or combinatorial TLR agonist and MEK1/2 inhibitor in wild-type and Irf1-/- macrophages. Results provide insights into the versatile but yet uncharacterized roles of IRF1 in TLR-MEK1/2-ERK MAPK signaling.

2018-12-28 | GSE123512 | GEO

Tracing the lineage of replicative RNAs carrying MEK1 in the evolution of drug resistance

Project description:Drug resistance poses a significant clinical challenge, and comprehending the mechanisms underlying this resistance can facilitate the design of novel inhibitors and advance cancer treatment. The REPLACE system was employed to examine resistance mutations in MEK1 during the administration of 3 allosteric inhibitors. To explore the accumulation of mutations in various RNAs during the MEK1 evolution experiment, we established a barcoded library and conducted lineage tracing of replicative RNAs carrying MEK1 throughout the drug resistance evolution process.

2024-09-30 | GSE271875 | GEO

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