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STAT1 transcription factor in Human HeLa S3

ABSTRACT: We report the results of chromatin immunoprecipitation following by high-thoughput tag sequencing (ChIP-Seq) using the GA II platform from Illumina for the human transcription factor STAT1 in HeLa S3 cells. The STAT1 ChIP was performed using HeLa S3 cells that are stimulated using gamma-interferon. We have also generated a seqenced input DNA dataset for gamma-interferon stimulated HeLa S3 cells. Raw data for this study is available for download from the Short Read Archive database at: http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?study=SRP000703. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Examination of the STAT1 transcription factor in Human HeLa S3.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Wilson

PROVIDER: E-GEOD-12782 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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PeakSeq enables systematic scoring of ChIP-seq experiments relative to controls.

Rozowsky Joel J Euskirchen Ghia G Auerbach Raymond K RK Zhang Zhengdong D ZD Gibson Theodore T Bjornson Robert R Carriero Nicholas N Snyder Michael M Gerstein Mark B MB

Nature biotechnology 20090104 1

Chromatin immunoprecipitation (ChIP) followed by tag sequencing (ChIP-seq) using high-throughput next-generation instrumentation is fast, replacing chromatin immunoprecipitation followed by genome tiling array analysis (ChIP-chip) as the preferred approach for mapping of sites of transcription-factor binding and chromatin modification. Using two deeply sequenced data sets for human RNA polymerase II and STAT1, each with matching input-DNA controls, we describe a general scoring approach to addre ...[more]

PMID: 19122651

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PolII transcription factor in Human HeLa S3

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2008-09-16 | E-GEOD-12781 | biostudies-arrayexpress

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Role for ISGF3II in the Antiviral Activity of IFN-gamma

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