Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

PolII transcription factor in Human HeLa S3

ABSTRACT: We report the results of chromatin immunoprecipitation following by high-thoughput tag sequencing (ChIP-Seq) using the GA II platform from Illumina for the human transcription factor PolII in HeLa S3 cells. The PolII ChIP was performed according to the same protocol as used for the STAT1 samples except that the cells were not stimulated using gamma-interferon prior to formaldehyde fixation. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Examination of the PolII transcription factor in Human HeLa S3.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Wilson 

PROVIDER: E-GEOD-12781 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Mapping accessible chromatin regions using Sono-Seq.

Auerbach Raymond K RK   Euskirchen Ghia G   Rozowsky Joel J   Lamarre-Vincent Nathan N   Moqtaderi Zarmik Z   Lefrançois Philippe P   Struhl Kevin K   Gerstein Mark M   Snyder Michael M  

Proceedings of the National Academy of Sciences of the United States of America 20090818 35

Disruptions in local chromatin structure often indicate features of biological interest such as regulatory regions. We find that sonication of cross-linked chromatin, when combined with a size-selection step and massively parallel short-read sequencing, can be used as a method (Sono-Seq) to map locations of high chromatin accessibility in promoter regions. Sono-Seq sites frequently correspond to actively transcribed promoter regions, as evidenced by their co-association with RNA Polymerase II Ch  ...[more]

PMID: 19706456

Publication: 1/2

Similar Datasets

Genomics STAT1 transcription factor in Human HeLa S3
2008-09-15 | E-GEOD-12782 | biostudies-arrayexpress
Genomics Genome-wide mapping of RNA Pol II binding site (GM12878)
2009-12-31 | E-GEOD-19550 | biostudies-arrayexpress
Genomics Genome-wide mapping of RNA Pol III binding site (K562)
2009-12-31 | E-GEOD-19548 | biostudies-arrayexpress
Genomics Genome-wide mapping of RNA Pol III binding site (GM12878)
2009-12-31 | E-GEOD-19549 | biostudies-arrayexpress
Genomics Genome-wide mapping of Jun binding site (K562)
2009-12-31 | E-GEOD-19545 | biostudies-arrayexpress
Genomics Genome-wide mapping of Myc binding site (K562)
2009-12-31 | E-GEOD-19547 | biostudies-arrayexpress
Genomics Genome-wide mapping of Fos binding site (K562)
2009-12-31 | E-GEOD-19546 | biostudies-arrayexpress
Genomics Genome-wide mapping of RNA Polymerase II binding sites
2008-10-02 | E-GEOD-13008 | biostudies-arrayexpress
Genomics Mapping Novel Chromatin Regions Using Sono-Seq
2008-12-18 | E-GEOD-14022 | biostudies-arrayexpress
Transcriptomics Integrative AUF1 PAR-CLIP analysis uncovers AUF1 roles in translation and genome integrity (RNA-Seq 2)
2014-11-04 | E-GEOD-52975 | biostudies-arrayexpress