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Gene expression profiling of human anaplastic thyroid carcinoma cells by microarray

ABSTRACT: In this study, we profiled the change of gene expression by p53 knock-down on anaplastic thyroid carcinoma (ATC) cell line, KAT-18 cells using Applied Biosystem's Microarrays. When compared with the untreated control KAT-18 cells, 2,926 genes were found to have altered expression levels of 2-fold or more in cells treated with antisense p53 oligonucleotide. Microarray analysis revealed that p53 knock-down modified the expression of numerous apoptosis-related genes. Among those genes, 33 apoptosis pathway activators and 20 apoptosis pathway repressors were identified. We analyzed human thyroid carcinoma cell (KAT18) on Applied Biosystem Human 1700 Chip Arrays. There were control and test sample, respectively, KAT18 untreated and treated antisense p53.

ORGANISM(S): Homo sapiens

SUBMITTER: Young Yoo

PROVIDER: E-GEOD-12912 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mutant p53 (G199V) gains antiapoptotic function through signal transducer and activator of transcription 3 in anaplastic thyroid cancer cells.

Kim Tae-Hyun TH Lee Sang Yull SY Rho Jee Hyun JH Jeong Na Young NY Soung Young Hwa YH Jo Wol Soon WS Kang Do-Young DY Kim Sung-Heun SH Yoo Young Hyun YH

Molecular cancer research : MCR 20091013 10

In the present study, we identified a missense mutation (G199V) in KAT-18 cell line established from primary cultures of anaplastic thyroid cancer (ATC). Notably, knockdown of this mutant (mt) p53 reduced cell viability and exerted antitumor activity equivalent to high doses of several chemotherapeutic agents. We showed that p53 knockdown had an antitumor effect via the induction of apoptosis. We further examined the underlying mechanism by which mt p53 (G199V) gains antiapoptotic function in KA ...[more]

PMID: 19825993

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