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MiR-K5 expression and inhibition

ABSTRACT: Transcriptional profiling of BJAB cells expressing miR-K5 and BCBL cells treated with miR-K5 inhibitor. To identify host RNA targets of KSHV miRNAs, we took advantage of the observation that RNAs targeted by miRNAs often display small reductions in their steady-state levels, perhaps as a result of their impaired translation. Accordingly, we examined cellular transcript accumulation by array-based expression profiling under four sets of conditions in which KSHV miRNAs were expressed or inhibited. Keywords: Experimental treatment versus control treatment Cells transfected with negative control miRNA compared to miR-K5 or negative miRNA inhibitor compared to miR-K5 inhibitor or stable cells transduced with empty vector compared to stable cells transduced to express miR cluster K1,2,3,4,5.

ORGANISM(S): Homo sapiens

SUBMITTER: Don Ganem

PROVIDER: E-GEOD-12967 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tandem array-based expression screens identify host mRNA targets of virus-encoded microRNAs.

Ziegelbauer Joseph M JM Sullivan Christopher S CS Ganem Don D

Nature genetics 20081221 1

MicroRNAs (miRNAs) are short noncoding RNAs of cellular and viral origin that post-transcriptionally regulate gene expression through imperfect base pairing to their mRNA targets. Because the recognition sequences of miRNAs for their targets are short and may be discontinuous, bioinformatic prediction of targets is difficult. Here we present an approach to the experimental identification of the mRNA targets of miRNAs encoded by the Kaposi's sarcoma-associated herpesvirus (KSHV). KSHV encodes 17 ...[more]

PMID: 19098914

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