Study of keratinocyte differentiation in normal human epidermis

ABSTRACT: In epidermis, granular keratinocytes are responsible for the synthesis of a great number of proteins of high tissue-specificity, and required for the epidermal barrier establishment and the regulation of the desquamation process. This project aims at characterizing the physiopathological function of genes implicated in these processes, and in the short term, defining their expression level in the course of keratinocyte differentiation. Pangenomic oligonucleotide microarrays are used to compare their expression between two cell populations enriched in basal or granular keratinocytes (T1 and T4 fractions resp.), obtained by successive incubations in trypsin solution of six independent normal human epidermis samples. Real-time quantitative PCR experiments were further performed to ensure the

ORGANISM(S): Homo sapiens

SUBMITTER: Nicolas Mattiuzzo 

PROVIDER: E-GEOD-13263 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress