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Transcription profiling of human leukocytes or whole blood isolated from burn/trauma subjects using either buffy coat or PAXgene methods

ABSTRACT: #G017 Burn/Trauma Study. This is a Glue Grant Study comparison between gene expression analyses performed in eight trauma/burn subjects using either a buffy coat isolation of whole blood or using the PAXgene system

ORGANISM(S): Homo sapiens

SUBMITTER: Lyle Moldawer

PROVIDER: E-GEOD-1343 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Whole blood and leukocyte RNA isolation for gene expression analyses.

Feezor Robert J RJ Baker Henry V HV Mindrinos Michael M Hayden Doug D Tannahill Cynthia L CL Brownstein Bernard H BH Fay Adrian A MacMillan Sandra S Laramie Jason J Xiao Wenzhong W Moldawer Lyle L LL Cobb J Perren JP Laudanski Krzysztof K Miller-Graziano Carol L CL Maier Ronald V RV Schoenfeld David D Davis Ronald W RW Tompkins Ronald G RG

Physiological genomics 20041101 3

The analysis of gene expression data in clinical medicine has been plagued by the lack of a critical evaluation of accepted methodologies for the collection, processing, and labeling of RNA. In the present report, the reliability of two commonly used techniques to isolate RNA from whole blood or its leukocyte compartment was compared by examining their reproducibility, variance, and signal-to-noise ratios. Whole blood was obtained from healthy subjects and was either untreated or stimulated ex v ...[more]

PMID: 15548831

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Burn/Trauma Study

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