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Differential gene expression in S284Y flies

ABSTRACT: RNA were extracted from 5 adult male and 5 adult female dU2AF50 wt and mutant flies grown at either the permisive (25C) or restrictive (30C) temperature for 4 days. Keywords: other

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Marco Blanchette

PROVIDER: E-GEOD-1345 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analysis reveals an unexpected function for the Drosophila splicing factor U2AF50 in the nuclear export of intronless mRNAs.

Blanchette Marco M Labourier Emmanuel E Green Richard E RE Brenner Steven E SE Rio Donald C DC

Molecular cell 20040601 6

The protein factor U2AF is an essential component required for pre-mRNA splicing. Mutations identified in the S. pombe large U2AF subunit were used to engineer transgenic Drosophila carrying temperature-sensitive U2AF large subunit alleles. Mutant recombinant U2AF heterodimers showed reduced polypyrimidine tract RNA binding at elevated temperatures. Genome-wide RNA profiling comparing wild-type and mutant strains identified more than 400 genes differentially expressed in the dU2AF50 mutant flies ...[more]

PMID: 15200955

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Differential gene expression in S284Y flies

Project description:RNA were extracted from 5 adult male and 5 adult female dU2AF50 wt and mutant flies grown at either the permisive (25C) or restrictive (30C) temperature for 4 days. Keywords: other

2004-05-25 | GSE1345 | GEO

RNA-seq analyses of kdm5 mutant flies and wt flies under normal condition and oxidative stress

Project description:Comparing the transcriptome of wildtype and kdm5 mutant flies in normal conditions revealed a total of 4787 genes that were significantly downregulated and thus require KDM5 for their activation, and 3269 upregulated genes that are normally repressed by KDM5 (p<0.05, FDR <0.05). Because kdm5 mutants are sensitive to the oxidizer paraquat, we also carried out RNA-seq from wildtype and kdm5 mutant adults in oxidative stress conditions. Paraquat treatment of wildtype flies lead to the upregulation of 2481, and downregulation of 3103 genes adult mRNA profiles of 1-3-days old wild type (WT) and kdm5 mutant under normal condition and oxitative stress were generated by deep sequencing, using Illumina HisSeq 2000.

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2012-12-06 | E-GEOD-42758 | biostudies-arrayexpress

C. elegans heat stress time course

Project description:We sought to study the effect of heat stress on gene expression in C. elegans. We cultured CF512 animals to adulthood at 25C (at which they are sterile). We then induced heat stress by switching the culture temperature to 30C. Keywords: time-course

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The foraging gene in Drosophila mediates metabolic as well as behavioral plasticity

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