Unknown,Transcriptomics,Genomics,Proteomics

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Gene Expression in Human Lung Type II Cells

ABSTRACT: Normal lung function relies on mature function of alveolar type II cels, which have numerous functions including to regulate ion and fluid flux, produce immune molecules, and synthesize and secrete surfactant to stabilize air spaces. Differentiation of type II cells from precursor epithelial cells is accelerated by exposure of cultured cells to glucocorticoid and cAMP. In these studies we used DNA microarray analysis to identify genes of both fetal and adult type II cells that are regulated by glucocorticoid plus cAMP. In the first series of experiments we isolated epithelial cells from fetal human lung and cultured cells for 5 days with or without hormone treatment (dexamethasone plus 8-Br-cAMP plus isobutylmethylxanthine, DCI) and then performed DNA microarray analysis. In the second series of experiments, we isolated type II cells from adult human postmortem lung and cultured cells for 5 days with or without DCI and performed DNA microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Philip Ballard

PROVIDER: E-GEOD-19699 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Regulated gene expression in cultured type II cells of adult human lung.

Ballard Philip L PL Lee Jae W JW Lee Jae W JW Fang Xiaohui X Chapin Cheryl C Allen Lennell L Segal Mark R MR Fischer Horst H Illek Beate B Gonzales Linda W LW Kolla Venkatadri V Matthay Michael A MA

American journal of physiology. Lung cellular and molecular physiology 20100409 1

Alveolar type II cells have multiple functions, including surfactant production and fluid clearance, which are critical for lung function. Differentiation of type II cells occurs in cultured fetal lung epithelial cells treated with dexamethasone plus cAMP and isobutylmethylxanthine (DCI) and involves increased expression of 388 genes. In this study, type II cells of human adult lung were isolated at approximately 95% purity, and gene expression was determined (Affymetrix) before and after cultur ...[more]

PMID: 20382749

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Dataset Information

Gene Expression in Human Lung Type II Cells

Publications

Regulated gene expression in cultured type II cells of adult human lung.

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