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Profiling 11,639 genes in PBMCs from 2 donors: U133 A 2.0 Custom CDF Version 9

ABSTRACT: The immune responses generated by YF-17D by profiling 11,639 genes in PBMCs from 2 donors cultured with YF-17D vaccine were accessed after 3 and 12 hours. Keywords: time course, in vitro stimulaion PMBCs were isolated with citrate buffered cell preparation tubes (CPT) from 2 healthy donors who were not previously vaccinated with YF-17D. Total RNA was either isolated directly from the freshly collected cells or after cell culture. Cells were cultured for 3 and 12 hours in the presence or absence of YF-17D vaccine.

ORGANISM(S): Homo sapiens

SUBMITTER: Vencio Ricardo

PROVIDER: E-GEOD-13484 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systems biology approach predicts immunogenicity of the yellow fever vaccine in humans.

Querec Troy D TD Akondy Rama S RS Lee Eva K EK Cao Weiping W Nakaya Helder I HI Teuwen Dirk D Pirani Ali A Gernert Kim K Deng Jiusheng J Marzolf Bruz B Kennedy Kathleen K Wu Haiyan H Bennouna Soumaya S Oluoch Herold H Miller Joseph J Vencio Ricardo Z RZ Mulligan Mark M Aderem Alan A Ahmed Rafi R Pulendran Bali B

Nature immunology 20081123 1

A major challenge in vaccinology is to prospectively determine vaccine efficacy. Here we have used a systems biology approach to identify early gene 'signatures' that predicted immune responses in humans vaccinated with yellow fever vaccine YF-17D. Vaccination induced genes that regulate virus innate sensing and type I interferon production. Computational analyses identified a gene signature, including complement protein C1qB and eukaryotic translation initiation factor 2 alpha kinase 4-an orche ...[more]

PMID: 19029902

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Project description:The immune responses generated by YF-17D by profiling 20,077 genes in 25 vaccine recipients were accessed at days 1, 3, 7, and 21 post-vaccination compared to pre-vaccination in PBMCs. The immune responses generated by YF-17D by profiling 20,077 genes in 25 vaccine recipients were accessed at days 1, 3, 7, and 21 post-vaccination compared to pre-vaccination in PBMCs. Keywords: time course This study is a combination of 2 separate YF-17D vaccination trials. Trial 1 has 15 subjects, and Trial 2 has 10 subjects. The two trials occurred 1 year apart and used different lots of the vaccine. Enrolled volunteers were healthy, aged 18 to 45, and signed a written informed consent form. Potential volunteers were excluded from participating in the study if they were pregnant, or if they had been vaccinated previously with YF-17D. Blood samples were taken pre-vaccinaion to serve as a reference point for each individual, and post-vaccination samples were taken a days 1, 3, 7, and 21 after YF-17D vaccination. All subjects received YF-17D. Blood samples for microarray analysis were collected either in blue and black topped citrate buffered cell preparation tubes (CPT). Following PMBC isolation from CPT, 2 x 106 cells were lysed in 1 ml of TRIzol and stored at -80?C (Cat# 15596-026; Invitrogen Life Technologies). After all time points were collected for a subject, the samples were thawed, and the RNA isolation proceeded according to the manufacturer’s protocol.

2008-11-22 | E-GEOD-13485 | biostudies-arrayexpress

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